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Nano Lett., Jan 29 2024, | https://doi.org/10.1021/acs.nanolett.3c04615 Plasmonic Optical Wells-Based Enhanced Rate PCR

Seungyeon Han,^1‡ Hyun Ji An,^1,2‡ Taejin Kwak,^3‡ Miseol Kim¹, Dongchoul Kim,^3* Luke P. Lee,^2,4,5,6,7* and Inhee Choi^1,8*

¹Department of Life Science, University of Seoul, Seoul, 02504, Republic of Korea.

²Harvard Medical School, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts 02115, United States.

³Department of Mechanical Engineering, Sogang University, Seoul, 04107, Republic of Korea.

⁴Department of Bioengineering, University of California, Berkeley, California 94720, United States.

⁵Department of Electrical Engineering and Computer Science, University of California, Berkeley, California 94720, United States.

⁶Institute of Quantum Biophysics, Department of Biophysics, Sungkyunkwan University, Suwon, 16419, Republic of Korea.

⁷Department of Chemistry & Nanoscience, Ewha Womans University, Seoul 03760, Republic of Korea.

⁸Department of Applied Chemistry, University of Seoul, Seoul, 02504, Republic of Korea.

^‡S.H., H.J.A., and T.K. contributed equally to this manuscript.

^*Corresponding Authors : Dongchoul Kim, Luke P. Lee, Inhee Choi

Abstract

Rapid, sensitive, inexpensive point-of-care molecular diagnostics are crucial for the efficient control of spreading viral diseases and biosecurity of global health. However, the gold standard, polymerase chain reaction (PCR) is time-consuming and expensive and needs specialized testing laboratories. Here, we report a low-cost yet fast, selective, and sensitive Plasmonic Optical Wells-Based Enhanced Rate PCR: POWER-PCR. We optimized the efficient optofluidic design of 3D plasmonic optical wells via the computational simulation of light-to-heat conversion and thermophoretic convection in a self-created plasmonic cavity. The POWER-PCR chamber with a self-passivation layer can concentrate incident light to accumulate molecules, generate rapid heat transfer and thermophoretic flow, and minimize the quenching effect on the naked Au surface. Notably, we achieved swift photothermal cycling of nucleic acid amplification in POWER-PCR on-a-chip in 4 min 24 s. The POWER-PCR will provide an excellent solution for affordable and sensitive molecular diagnostics for precision medicine and preventive global healthcare.

논문정보

형식Research article
게재일2024년 01월 (BRIC 등록일 2024-02-01)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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