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- 조회636
Proc. Natl. Acad. Sci. USA, Feb 06 2024, 121 (6) | https://doi.org/10.1073/pnas.2317461121
A large-effect fitness trade-off across environments is explained by a single mutation affecting cold acclimation
Gwonjin Lee 1,2, Brian J Sanderson 1,2, Thomas J Ellis 3, Brian P Dilkes 2,4, John K McKay 5, Jon Ågren 3, Christopher G Oakley 1,2
1Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907.
2Center for Plant Biology, Purdue University, West Lafayette, IN 47907.
3Plant Ecology and Evolution, Department of Ecology and Genetics, Evolutionary Biology Centre, Uppsala University, Uppsala SE-752 36, Sweden.
4Department of Biochemistry, Purdue University, West Lafayette, IN 47907.
5Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO 80523.
To whom correspondence may be addressed.: Christopher G Oakley
Abstract
Identifying the genetic basis of local adaptation and fitness trade-offs across environments is a central goal of evolutionary biology. Cold acclimation is an adaptive plastic response for surviving seasonal freezing, and costs of acclimation may be a general mechanism for fitness trade-offs across environments in temperate zone species. Starting with locally adapted ecotypes of
Arabidopsis thaliana from Italy and Sweden, we examined the fitness consequences of a naturally occurring functional polymorphism in
CBF2. This gene encodes a transcription factor that is a major regulator of cold-acclimated freezing tolerance and resides within a locus responsible for a genetic trade-off for long-term mean fitness. We estimated the consequences of alternate genotypes of
CBF2 on 5-y mean fitness and fitness components at the native field sites by comparing near-isogenic lines with alternate genotypes of
CBF2 to their genetic background ecotypes. The effects of
CBF2 were validated at the nucleotide level using gene-edited lines in the native genetic backgrounds grown in simulated parental environments. The foreign
CBF2 genotype in the local genetic background reduced long-term mean fitness in Sweden by more than 10%, primarily via effects on survival. In Italy, fitness was reduced by more than 20%, primarily via effects on fecundity. At both sites, the effects were temporally variable and much stronger in some years. The gene-edited lines confirmed that
CBF2 encodes the causal variant underlying this genetic trade-off. Additionally, we demonstrated a substantial fitness cost of cold acclimation, which has broad implications for potential maladaptive responses to climate change.
논문정보
- Research article
- 2024년 02월 (BRIC 등록일 2024-02-01)
- 국외 연구진
- 생명과학 > 유전학
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