한빛사
>
한빛사논문
한빛사논문
- 조회509
Biosens. Bioelectron., Jan 19 2024, 250 S0956-5663(24)00058-7 | https://doi.org/10.1016/j.bios.2024.116055
Tetrahedral DNA nanostructures enhance transcription isothermal amplification for multiplex detection of non-coding RNAs
Eun Sung Lee, Jisu Woo, Jiye Shin, Byung Seok Cha, Seokjoon Kim, Ki Soo Park
Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, Republic of Korea
Corresponding author : Ki Soo Park
Abstract
This study introduces an innovative detection system for multiple cancer biomarkers, employing transcription isothermal amplification methods in conjunction with a tetrahedral DNA nanostructure (TDN). We demonstrate that TDN enhances various transcription isothermal amplification methods by placing DNA probes in proximity. Notably, the TDN-enhanced split T7 promoter-based isothermal transcription amplification with light-up RNA aptamer (STAR) system stands out for its optimal performance and operational simplicity, especially in identifying non-coding RNAs such as microRNAs and long non-coding RNAs (lncRNAs). Multiplex detection of lncRNAs was also achieved by generating distinct light-up RNA aptamers, each emitting unique fluorescence signals. The system effectively identified the target lncRNAs, demonstrating high sensitivity and selectivity in both cell lines and clinical samples. The system, utilizing the single enzyme T7 RNA polymerase, can be easily tailored for alternative targets by substituting target-specific sequences in DNA probes and seamlessly integrated with other isothermal amplification methods for greater sensitivity and accuracy in the detection of multiple cancer biomarkers.
논문정보
- Research article
- 2024년 01월 (BRIC 등록일 2024-01-26)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
댓글 작성 로그인
팝업 닫기관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기
