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지창훈Chang-Hun Ji

건국대학교

Nat. Prod. Rep., Jan 23 2024, | https://doi.org/10.1039/d3np00049d Promoter engineering of natural product biosynthetic gene clusters in actinomycetes: concepts and applications

Chang-Hun Ji,^†a Hyun-Woo Je,^†a Hiyoung Kim^†a and Hahk-Soo Kang^*a

aDepartment of Biomedical Science and Engineering, Konkuk University, Seoul 05029, Korea

† These authors equally contributed to this work.

*Corresponding authors

Abstract

Covering 2011 to 2022Low titers of natural products in laboratory culture or fermentation conditions have been one of the challenging issues in natural products research. Many natural product biosynthetic gene clusters (BGCs) are also transcriptionally silent in laboratory culture conditions, making it challenging to characterize the structures and activities of their metabolites. Promoter engineering offers a potential solution to this problem by providing tools for transcriptional activation or optimization of biosynthetic genes. In this review, we summarize the 10 years of progress in promoter engineering approaches in natural products research focusing on the most metabolically talented group of bacteria actinomycetes.

논문정보

형식Review Paper
게재일2024년 01월 (BRIC 등록일 2024-02-16)
연구진국내 연구진
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