한빛사논문
- 조회956
Yoonhee Lee 1,2,7, Jakob Buchheim 1,3,7, Björn Hellenkamp 1, David Lynall 1, Kyungae Yang 4, Erik F. Young 5, Boyan Penkov 1, Samuel Sia 6, Milan N. Stojanovic 4 & Kenneth L. Shepard 1,6,*
1Department of Electrical Engineering, Columbia University, New York, NY, USA.
2Division of Electronics & Information System, ICT Research Institute, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, Republic of Korea.
3Deutsches Zentrum für Luft- und Raumfahrt e.V. (DLR), Institute of Quantum Technologies, Ulm, Germany.
4Department of Medicine, Columbia University, New York, NY, USA.
5Quicksilver Biosciences, Inc., New York, NY, USA.
6Department of Biomedical Engineering, Columbia University, New York, NY, USA.
7These authors contributed equally: Yoonhee Lee, Jakob Buchheim.
*Corresponding author: correspondence to Kenneth L. Shepard
Abstract
Small molecules such as neurotransmitters are critical for biochemical functions in living systems. While conventional ultraviolet–visible spectroscopy and mass spectrometry lack portability and are unsuitable for time-resolved measurements in situ, techniques such as amperometry and traditional field-effect detection require a large ensemble of molecules to reach detectable signal levels. Here we demonstrate the potential of carbon-nanotube-based single-molecule field-effect transistors (smFETs), which can detect the charge on a single molecule, as a new platform for recognizing and assaying small molecules. smFETs are formed by the covalent attachment of a probe molecule, in our case a DNA aptamer, to a carbon nanotube. Conformation changes on binding are manifest as discrete changes in the nanotube electrical conductance. By monitoring the kinetics of conformational changes in a binding aptamer, we show that smFETs can detect and quantify serotonin at the single-molecule level, providing unique insights into the dynamics of the aptamer–ligand system. In particular, we show the involvement of G-quadruplex formation and the disruption of the native hairpin structure in the conformational changes of the serotonin–aptamer complex. The smFET is a label-free approach to analysing molecular interactions at the single-molecule level with high temporal resolution, providing additional insights into complex biological processes.
논문정보
- Research article
- 2024년 01월 (BRIC 등록일 2024-01-18)
- 국내+국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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