한빛사논문
- 조회540
Jun Young Hwang1, Kyung Ho Kim2,3, Sung Eun Seo3,4, Youngju Nam1, Sanghee Jwa1, Inwoo Yang1, Tai Hyun Park5, Oh Seok Kwon6,7, Seung Hwan Lee1
1Department of Bionano Engineering, Center for Bionano Intelligence Education and Research, Hanyang University, 15588 Ansan, Republic of Korea
2Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141 Republic of Korea
3Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 34141 125 Gwahak-ro, Yuseong-gu, Daejeon, Republic of Korea
4Department of Civil and Environmental Engineering, Yonsei University, Seoul, 03722 Republic of Korea
5Department of Nutritional Science and Food Management, Ewha Womans University, 03760 Seoul, Republic of Korea
6Department of Nano Science and Technology, Sungkyunkwan University, 16419 Suwon, Republic of Korea
7SKKU Advanced Institute of Nanotechnology (SAINT) Department of Nano Engineering, Sungkyunkwan University, 16419 Suwon, Republic of Korea
J.Y.H., K.H.K., and S.E.S. contributed equally to this work.
CORRESPONDING AUTHORS : Tai Hyun Park, Oh Seok Kwon, Seung Hwan Lee
Abstract
Bitterness elicits unpleasant sensations in humans, which can hinder the acceptance of foods and medication adherence. Therefore, identifying and masking bitter tastes is crucial for developing palatable foods and promoting medication compliance in the food and pharmaceutical industries. To achieve this, employing agonism and antagonism of bitter taste receptors as effective strategies at the molecular level is essential. In this study, a bioelectronic tongue is developed to characterize the agonism and antagonism of bitter taste receptors. The human bitter taste receptors hTAS2R16 and hTAS2R31 are produced using an Escherichia coli expression system and reconstituted into nanodiscs (NDs). Subsequently, hTAS2R16- and hTAS2R31-NDs are immobilized on the surface of graphene field-effect transistors (FETs) to construct bioelectronic tongues. The developed system sensitively detected the bitter agonists, salicin and saccharin, at concentrations as low as 100 fM, with high selectivity in real-time. The dose-dependent curves shifted and K values decreased by the antagonists of hTAS2R16 and hTAS2R31, indicating antagonism-based masking of bitter taste. Therefore, the developed bioelectronic tongue holds promise for identifying bitter tastes and evaluating the masking of bitterness based on the agonism and antagonism of hTAS2Rs.
논문정보
- Research article
- 2023년 12월 (BRIC 등록일 2024-01-17)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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