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한국표준과학연구원

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Nano Lett., Jan 12 2024, | https://doi.org/10.1021/acs.nanolett.3c02939 Novel Organoid Culture System for Improved Safety Assessment of Nanomaterials

Ahruem Baek ¹, Ik Hwan Kwon², Da-Hye Lee ³, Woo Hee Choi ^4,5, Sang-Won Lee ², Jongman Yoo ^4,5, Min Beom Heo ¹, Tae Geol Lee ¹

¹Nano-Safety Team, Safety Measurement Institute, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Republic of Korea.

²Bioimaging Team, Safety Measurement Institute, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Republic of Korea.

³Biomolecular Measurement Team, Bio-Metrology Group, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Republic of Korea.

⁴Department of Microbiology, CHA University School of Medicine, Seongnam 13488, Republic of Korea.

⁵Organoidsciences Ltd., Seongnam 13488, Republic of Korea.

Corresponding Authors: Min Beom Heo, Tae Geol Lee

Abstract

Over the past few decades, the increased application of nanomaterials has raised questions regarding their safety and possible toxic effects. Organoids have been suggested as promising tools, offering efficient assays for nanomaterial-induced toxicity evaluation. However, organoid systems have some limitations, such as size heterogeneity and poor penetration of nanoparticles because of the extracellular matrix, which is necessary for organoid culture. Here, we developed a novel system for the improved safety assessment of nanomaterials by establishing a 3D floating organoid paradigm. In addition to overcoming the limitations of two-dimensional systems including the lack of in vitro- in vivo cross-talk, our method provides multiple benefits as compared with conventional organoid systems that rely on an extracellular matrix for culture. Organoids cultured using our method exhibited relatively uniform sizing and structural integrity and were more conducive to the internalization of nanoparticles. Our floating culture system will accelerate the research and development of safe nanomaterials.

논문정보

형식Research article
게재일2024년 01월 (BRIC 등록일 2024-01-15)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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