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Bioresour. Technol., Volume 394, February 2024, 130304 | https://doi.org/10.1016/j.biortech.2024.130304
Direct and robust citramalate production from brown macroalgae using fast-growing Vibrio sp. dhg
Hye Kyung Lee a,1, Sunghwa Woo b,1, Dongyeop Baek b, Myeongwon Min a, Gyoo Yeol Jung a,b, Hyun Gyu Lim c
aSchool of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 37673, Korea
bDepartment of Chemical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 37673, Korea
cDepartment of Biological Engineering, Inha University, 100 Inha-Ro, Michuhol-Gu, Incheon 22212, Korea
1These authors made equal contributions to this work.
Corresponding authors : Gyoo Yeol Jung, Hyun Gyu Lim
Abstract
Brown macroalgae is a promising feedstock for biorefinery owing to its high biomass productivity and contents of carbohydrates such as alginate and mannitol. However, the limited availability of microbial platforms efficiently catabolizing the brown macroalgae sugars has restricted its utilization. In this study, the direct production of citramalate, an important industrial compound, was demonstrated from brown macroalgae by utilizing Vibrio sp. dhg, which has a remarkably efficient catabolism of alginate and mannitol. Specifically, citramalate synthase from Methanocaldococcus jannaschii was synthetically expressed, and competing pathways were removed to maximally redirect the carbon flux toward citramalate production. Notably, a resulting strain, VXHC, produced citramalate up to 9.8 g/L from a 20 g/L mixture of alginate and mannitol regardless of their ratios. Citramalate was robustly produced even when diverse brown macroalgae were provided directly. Collectively, this study showcased the high potential of brown macroalgae biorefinery using Vibrio sp. dhg.
논문정보
- Research article
- 2024년 02월 (BRIC 등록일 2024-01-15)
- 국내 연구진
- 생명과학 > 생물공학
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