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조회268

이요한Yohan Lee

KAIST

Nano Lett., Jan 07 2024, | https://doi.org/10.1021/acs.nanolett.3c03976 Structural Determinants of Chirally Selective Transport of Amino Acids through the α-Hemolysin Protein Nanopores of Free-Standing Planar Lipid Membranes

Yohan Lee¹, Sanggyu Chong¹, Chiwoo Lee², Jihan Kim ¹, Siyoung Q Choi ¹

¹Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.

²Chiral Technology Korea (CTK), Daejeon 34013, Republic of Korea.

Y.L. and S.C. contributed equally to this work.

Corresponding Author : Siyoung Q. Choi

Abstract

Despite the importance of the enantioselective transport of amino acids through transmembrane protein nanopores from fundamental and practical perspectives, little has been explored to date. Here, we study the transport of amino acids through α-hemolysin (αHL) protein pores incorporated into a free-standing lipid membrane. By measuring the transport of 13 different amino acids through the αHL pores, we discover that the molecular size of the amino acids and their capability to form hydrogen bonds with the pore surface determine the chiral selectivity. Molecular dynamics simulations corroborate our findings by revealing the enantioselective molecular-level interactions between the amino acid enantiomers and the αHL pore. Our work is the first to present the determinants for chiral selectivity using αHL protein as a molecular filter.

논문정보

형식Research article
게재일2024년 01월 (BRIC 등록일 2024-01-23)
연구진국내 연구진
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