박필구 (가천대학교)

한빛사논문

조회476

박필구Pil-gu Park

가천대학교

CV File 121 kb

J. Med. Virol., Jan 2024, 96 (1) | https://doi.org/10.1002/jmv.29361 Saliva-based Proteinase K method: A rapid and reliable diagnostic tool for the detection of SARS-COV-2 in children

Min Jung Kim¹, Pil‐gu Park², Su Jin Hwang³, Seung Jun Bang⁴, Jae Hwa Jung⁵, Eun Bin Kown¹, Eun Kyung Sul¹, Kyung Chul Song¹, Joon‐sik Choi¹, Kyeo Re Han³, Hye Young Lee⁶, Dong Hyun Kim⁴, Jae Myun Lee³

¹Department of Pediatrics, Yongin Severance Hospital, Yonsei University College of Medicine, Yongin‐si, Korea

²Department of Microbiology, Gachon University, Incheon, Korea

³Department of Microbiology and Immunology, Institute for Immunology and Immunological Diseases, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea

⁴Department of Pediatrics, Inha University Hospital, Inha University College of Medicine, Incheon, Korea

⁵Department of Pediatrics, Severance Children's Hospital, Yonsei University Collegeof Medicine, Seoul, Korea

⁶Department of Biomedical Laboratory Science, College of Software and Digital Healthcare Convergence, Yonsei University, Wonju, Korea

Min Jung Kim and Pil-gu Park are contributed equally to this work.

Correspondence : Dong Hyun Kim, Jae Myun Lee

Abstract

Early and accurate detection of viruses in children might help prevent transmission and severe diseases. In this study, the severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) detection in children was evaluated using saliva specimens with a Proteinase K (PTK)-based RNA preparation, as saliva collection is a simple and noninvasive procedure, even in young children, with fewer concerns about sample contamination. The saliva-based PTK and the conventional paired nasopharyngeal aspiration (NPA)-based detection methods were compared between COVID-19-positive and -negative children. In addition, the detection rate for SARS-COV-2 and the difference between admission and discharge by the saliva-based PTK method was tested in COVID-19 patients. The diagnostic accuracy of the saliva-based PTK method was 98.8% compared to NP swab-based reverse transcriptase polymerase chain reaction. Saliva samples showed high sensitivity (94.1%) and specificity (100%) when using the PTK method. Furthermore, the saliva-based PTK method significantly reduced the test processing time by 2 h. Notably, C _t values at discharge increased in saliva samples compared with those at admission, which might indicate patients' clinical conditions or virus activity. In conclusion, the saliva-based PTK implemented in this study streamlines RNA extraction, making the process faster, safer, and more cost-effective, demonstrating that this method is a rapid and reliable diagnostic tool for SARS-CoV-2 detection in children.

논문정보

형식Research article
게재일2024년 01월 (BRIC 등록일 2024-01-08)
연구진국내 연구진
분야 의약학 > 감염의학

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