박경민 (고려대학교 의과대학) | 한빛사논문 > 한빛사

한빛사논문

조회335

박경민Kyungmin Park

고려대학교 의과대학

CV File 91 kb

J. Med. Virol., Jan 2024, 96 (1) | https://doi.org/10.1002/jmv.29346 High-resolution phylogeographical surveillance of Hantaan orthohantavirus using rapid amplicon-based Flongle sequencing, Republic of Korea

Jongwoo Kim ^1,2, Kyungmin Park ^1,2, Kijin Kim ^3,4, Juyoung Noh ^1,2, Seong-Gyu Kim ^1,2, Eunyoung Yang ^1,2, Hee-Kyung Cho ^1,2, Seung-Ho Lee ⁵, Jin Sun No ⁶, Geum-Young Lee ¹, Daesang Lee ⁵, Dong-Hyun Song ⁵, Se Hun Gu ⁵, Man-Seong Park ^1,2,7, Nam-Hyuk Cho ^8,9, Seong Tae Jeong ⁵, Won-Keun Kim ^10,11, Jin-Won Song ^1,2

¹Department of Microbiology, Korea University College of Medicine, Seoul, Republic of Korea.

²BK21 Graduate Program, Korea University College of Medicine, Seoul, Republic of Korea.

³Centre for Infectious Disease Genomics and One Health, Faculty of Health Sciences, Simon Fraser University, Burnaby, British Columbia, Canada.

⁴Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada.

⁵Chem-Bio Technology Center, Agency for Defense Development, Daejeon, Republic of Korea.

⁶Division of High-Risk Pathogens, Bureau of Infectious Diseases Diagnosis Control, Korea Disease Control and Prevention Agency, Cheongju, Republic of Korea.

⁷Department of Microbiology, Institute for Viral Diseases, College of Medicine, Korea University, Seoul, Republic of Korea.

⁸Department of Microbiology and Immunology, College of Medicine, Seoul National University, Seoul, Republic of Korea.

⁹Department of Biomedical Sciences, College of Medicine, Seoul National University, Seoul, Republic of Korea.

¹⁰Department of Microbiology, Hallym University, Chuncheon, Republic of Korea.

¹¹Institute of Medical Research, Hallym University, Chuncheon, Republic of Korea.

Jongwoo Kim and Kyungmin Park contributed equally to this work.

CORRESPONDING AUTHOR : Jin-Won Song

Abstract

Orthohantaviruses, etiological agents of hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome, pose a critical public health threat worldwide. Hantaan orthohantavirus (HTNV) outbreaks are particularly endemic in Gyeonggi Province in northern area of the Republic of Korea (ROK). Small mammals were collected from three regions in the Gyeonggi Province during 2017 and 2018. Serological and molecular prevalence of HTNV was 25/201 (12.4%) and 10/25 (40%), respectively. A novel nanopore-based diagnostic assay using a cost-efficient Flongle chip was developed to rapidly and sensitively detect HTNV infection in rodent specimens within 3 h. A rapid phylogeographical surveillance of HTNV at high-resolution phylogeny was established using the amplicon-based Flongle sequencing. In total, seven whole-genome sequences of HTNV were newly obtained from wild rodents collected in Paju-si (Gaekhyeon-ri) and Yeoncheon-gun (Hyeonga-ri and Wangnim-ri), Gyeonggi Province. Phylogenetic analyses revealed well-supported evolutionary divergence and genetic diversity, enhancing the resolution of the phylogeographic map of orthohantaviruses in the ROK. Incongruences in phylogenetic patterns were identified among HTNV tripartite genomes, suggesting differential evolution for each segment. These findings provide crucial insights into on-site diagnostics, genome-based surveillance, and the evolutionary dynamics of orthohantaviruses to mitigate hantaviral outbreaks in HFRS-endemic areas in the ROK.

논문정보

형식Research article
게재일2024년 01월 (BRIC 등록일 2024-01-08)
연구진국내(교신)+국외 연구진
분야

댓글 작성 로그인

CV 열람하기

연락처 보기