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정영철Youngcheol Jung

도쿄대학교

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J. Am. Chem. Soc., Dec 06 2023, 145 (48) | https://doi.org/10.1021/jacs.3c10603 Function and Structure of a Terpene Synthase Encoded in a Giant Virus Genome

Youngcheol Jung,^† Takaaki Mitsuhashi,^*,‡ Sota Sato,^†,‡ Miki Senda,^# Toshiya Senda,^# and Makoto Fujita^*,†,‡,¶

^†Department of Applied Chemistry, School of Engineering, The University of Tokyo, Mitsui Link Lab Kashiwanoha 1, FS CREATION, 6-6-2 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan

^‡Division of Advanced Molecular Science, Institute for Molecular Science (IMS), Okazaki, Aichi 444-8787, Japan

^#Structural Biology Research Center, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), 1-1 Oho, Tsukuba, Ibaraki 305-0801, Japan

^¶Tokyo College, Institutes for Advanced Study, The University of Tokyo, Mitsui Link Lab Kashiwanoha 1, FS CREATION, 6-6-2 Kashiwanoha, Chiba 277-0882, Japan

*Corresponding Authors : Takaaki Mitsuhashi, Makoto Fujita

Abstract

Giant viruses are nonstandard viruses with large particles and genomes. While previous studies have shown that their genomes contain various sequences of interest, their genes related specifically to natural product biosynthesis remain unexplored. Here we analyze the function and structure of a terpene synthase encoded by the gene of a giant virus. The enzyme is phylogenetically separated from the terpene synthases of cellular organisms; however, heterologous gene expression revealed that it still functions as a terpene synthase and produces a cyclic terpene from a farnesyl diphosphate precursor. Crystallographic analysis revealed its protein structure, which is relatively compact but retains essential motifs of the terpene synthases. We thus suggest that like cellular organisms, giant viruses produce and utilize natural products for their ecological strategies.

논문정보

형식Research article
게재일2023년 12월 (BRIC 등록일 2024-01-08)
연구진국외 연구진
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