Shine Augustine (성균관대학교)

한빛사논문

조회341

Shine AugustineShine Augustine

성균관대학교

Biosens. Bioelectron., Dec 30 2023, 248 S0956-5663(23)00929-6 | https://doi.org/10.1016/j.bios.2023.115987 Metal-enhanced fluorescence biosensor integrated in capillary flow-driven microfluidic cartridge for highly sensitive immunoassays

Shine Augustine ^a,1, Mottour Vinayagam Chinnamani^a,1, Chae Won Mun ^b, Jeong-Yong Shin ^c, Tran Quang Trung ^a, Seok Ju Hong ^a, Lai Thi Ngoc Huyen ^a, Eung Hyuk Lee ^d, Soo Hyun Lee ^b, Jong-Joo Rha^b, Sunghoon Jung^b, Yunjong Lee ^c, Sung-Gyu Park ^b, Nae-Eung Lee ^a,d,e,f,g

^aSchool of Advanced Materials Science & Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do, 16419, Republic of Korea

^bNano-Bio Convergence Department, Korea Institute of Materials Science (KIMS), Changwon, 51508, Republic of Korea

^cDivision of Pharmacology, Department of Molecular Cell Biology, Samsung, Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Gyeonggi-do, 16419, Republic of Korea

^dSKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon, Gyeonggi-do, 16419, Republic of Korea

^eSamsung Advanced Institute for Health Sciences & Technology (SAIHST), Sungkyunkwan University, Suwon, Gyeonggi-do, 16419, Republic of Korea

^fInstitute of Quantum Biophysics (IQB), Sungkyunkwan University, Suwon, Gyeonggi-do, 16419, Republic of Korea

^gBiomedical Institute for Convergence at SKKU (BICS), Sungkyunkwa n University, Suwon, Gyeonggi-do, 16419, Republic of Korea

¹These authors contributed equally to this work.

Corresponding authors: Sung-Gyu Park, Nae-Eung Lee

Abstract

Point-of-care testing (POCT) for low-concentration protein biomarkers remains challenging due to limitations in biosensor sensitivity and platform integration. This study addresses this gap by presenting a novel approach that integrates a metal-enhanced fluorescence (MEF) biosensor within a capillary flow-driven microfluidic cartridge (CFMC) for the ultrasensitive detection of the Parkinson's disease biomarker, aminoacyl-tRNA synthetase complex interacting multi-functional protein 2 (AIMP-2). Crucial point to this approach is the orientation-controlled immobilization of capture antibody on a nanodimple-structured MEF substrate within the CFMC. This strategy significantly enhances fluorescence signals without quenching, enabling accurate quantification of low-concentration AIMP-2 using a simple digital fluorescence microscope with a light-emitting diode excitation source and a digital camera. The resulting platform exhibits exceptional sensitivity, achieving a limit of detection in the pg/mL range for AIMP-2 in human serum. Additionally, the CFMC design incorporates a capillary-driven passive sample transport mechanism, eliminating the need for external pumps and further simplifying the detection process. Overall, this work demonstrates the successful integration of MEF biosensing with capillary microfluidics for point-of-care applications.

논문정보

형식Research article
게재일2023년 12월 (BRIC 등록일 2024-01-15)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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