강문성 (부산대학교)

한빛사논문

조회425

부산대학교

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Nano-Micro Lett., Jan 04 2024, 16 (1) | https://doi.org/10.1007/s40820-023-01293-1 Highly Aligned Ternary Nanofiber Matrices Loaded with MXene Expedite Regeneration of Volumetric Muscle Loss

Moon Sung Kang¹, Yeuni Yu², Rowoon Park¹, Hye Jin Heo³, Seok Hyun Lee^1,8, Suck Won Hong^1,4*, Yun Hak Kim^2,5,6*, Dong-Wook Han^1,7*

¹Department of Cogno-Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan 46241, Republic of Korea

²Medical Research Institute, School of Medicine, Pusan National University, Yangsan 50612, Republic of Korea

³Department of Anatomy, School of Medicine, Pusan National University, Yangsan 50612, Republic of Korea

⁴Engineering Research Center for Color-Modulated Extra-Sensory Perception Technology, Pusan National University, Busan 46241, Republic of Korea

⁵Department of Biomedical Informatics, School of Medicine, Pusan National University, Yangsan 50612, Republic of Korea

⁶Periodontal Disease Signaling Network Research Center and Dental and Life Science Institute, School of Dentistry, Pusan National University, Yangsan 50612, Republic of Korea

⁷BIO-IT Fusion Technology Research Institute, Pusan National University

⁸Present Address: Osstem Implant Inc., Seoul 07789, Republic of Korea

Moon Sung Kang, Yeuni Yu, and Rowoon Park contributed equally to this work.

Corresponding authors : Correspondence to Suck Won Hong, Yun Hak Kim or Dong-Wook Han.

Abstract

Current therapeutic approaches for volumetric muscle loss (VML) face challenges due to limited graft availability and insufficient bioactivities. To overcome these limitations, tissue-engineered scaffolds have emerged as a promising alternative. In this study, we developed aligned ternary nanofibrous matrices comprised of poly(lactide-co-ε-caprolactone) integrated with collagen and Ti ₃C ₂T _x MXene nanoparticles (NPs) (PCM matrices), and explored their myogenic potential for skeletal muscle tissue regeneration. The PCM matrices demonstrated favorable physicochemical properties, including structural uniformity, alignment, microporosity, and hydrophilicity. In vitro assays revealed that the PCM matrices promoted cellular behaviors and myogenic differentiation of C2C12 myoblasts. Moreover, in vivo experiments demonstrated enhanced muscle remodeling and recovery in mice treated with PCM matrices following VML injury. Mechanistic insights from next-generation sequencing revealed that MXene NPs facilitated protein and ion availability within PCM matrices, leading to elevated intracellular Ca ²⁺ levels in myoblasts through the activation of inducible nitric oxide synthase (iNOS) and serum/glucocorticoid regulated kinase 1 (SGK1), ultimately promoting myogenic differentiation via the mTOR-AKT pathway. Additionally, upregulated iNOS and increased NO ^- contributed to myoblast proliferation and fiber fusion, thereby facilitating overall myoblast maturation. These findings underscore the potential of MXene NPs loaded within highly aligned matrices as therapeutic agents to promote skeletal muscle tissue recovery.

논문정보

형식Research article
게재일2024년 01월 (BRIC 등록일 2024-01-05)
연구진국내 연구진
분야 바이오·의료융합 > 바이오소재

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