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서울시립대학교

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J. Hazard. Mater., Dec 28 2023, 465 S0304-3894(23)02643-2 | https://doi.org/10.1016/j.jhazmat.2023.133359 Microplastic-induced inhibition of cell adhesion and toxicity evaluation using human dermal fibroblast-derived spheroids

Seonghyeon Eom ^a, Woosung Shim ^a, Inhee Choi ^a,b

^aDepartment of Life Science, University of Seoul, Seoul 02504, Republic of Korea

^bDepartment of Applied Chemistry, University of Seoul, Seoul 02504, Republic of Korea

Corresponding author : Inhee Choi

Abstract

Nanoplastics and microplastics (MPs) can significantly affect marine ecosystems and pose potential risks to human health. Although adverse effects stemming from direct exposure to MPs have been demonstrated at the cellular level in animal models, the potential toxicity of these materials in the human body remains uncertain. In this study, we investigated the three-dimensional (3D) behavior of dermal-derived cells exposed to MPs using artificially manufactured spherical primary polystyrene (PS) particles. To explore these effects, we used cellular spheroids as a 3D cell culture model, examined the size-dependent penetration of PS-MPs, and observed morphological alterations in the spheroids. Furthermore, we assessed changes in physiological activities, including reactive oxygen species, adenosine triphosphate, and lactate dehydrogenase, to elucidate the potential intra- and extracellular toxic reactions to PS-MPs. Additionally, our examination of cell-cell junctions and the extracellular matrix (ECM), along with analysis of the regulators involved in their decreased integrity, revealed negatively influenced changes in expression. This exposure study using spheroid models provides new insights into the potential toxicity of short-term exposure to MPs under conditions that closely resemble in vivo systems.

논문정보

형식Research article
게재일2023년 12월 (BRIC 등록일 2024-01-05)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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