김종호 (한양대학교)

한빛사논문

조회291

한양대학교

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Biosens. Bioelectron., Dec 20 2023, 248 S0956-5663(23)00890-4 | https://doi.org/10.1016/j.bios.2023.115948 Confined growth of Ag nanogap shells emitting stable Raman label signals for SERS liquid biopsy of pancreatic cancer

In-Jun Hwang ^a, Chanhee Choi ^a, Hongwon Kim^a, Hyunji Lee ^a, Yejoo Yoo ^a, Yujin Choi ^a, Jin-Hyeok Hwang ^b, Kwangrok Jung ^b, Jong-Chan Lee ^b, Jong-Ho Kim ^a

^aDepartment of Materials Science and Chemical Engineering, Hanyang University, Ansan, 15588, Republic of Korea

^bDepartment of Internal Medicine, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, 03080, Republic of Korea

Corresponding author : Jong-Ho Kim

Abstract

To develop a reliable surface-enhanced Raman scattering (SERS) immunoassay as a new liquid biopsy modality, SERS nanoprobes emitting strong and stable signals are necessary. However, Ag nanoparticles used as SERS nanoprobes are prone to rapid fading of SERS signals by oxidation. This has driven the development of a new strategy for Ag-based SERS nanoprobes emitting stable and strong SERS signals over time. Herein, Ag nanogap shells entrapping Raman labels are created in the confined pores of mesoporous silica nanoparticles (AgNSM) through a rapid single-step reaction for SERS liquid biopsy. Each AgNSM nanoprobe possesses multiple nanogaps of 1.58 nm to entrap Raman labels, allowing superior long-term SERS signal stability and large enhancement of 1.5 × 10 ⁶. AgNSM nanoprobes conjugated with an antibody specific for carbohydrate antigen (CA)19-9 are employed in the SERS sandwich immunoassay including antibody-conjugated magnetic nanoparticles for CA19-9 detection, showing a two orders of magnitude lower limit of detection (0.025 U mL ^-1) than an enzyme-linked immunosorbent assay (0.3 U mL ^-1). The AgNSM nanoprobe immunoassay accurately quantifies CA19-9 levels from clinical serum samples of early and advanced pancreatic cancer. AgNSM nanoprobes with stable SERS signals provide a new route to SERS liquid biopsy for effective detection of blood biomarkers.

논문정보

형식Research article
게재일2023년 12월 (BRIC 등록일 2024-01-05)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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