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University of Nebraska-Lincoln

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Adv. Funct. Mater., Volume33, Issue35 August 29, 2023 2214129 | https://doi.org/10.1002/adfm.202214129 A Facile Strategy for the Fabrication of Cell-laden Porous Alginate Hydrogels Based on Two-phase Aqueous Emulsions

Wen Xue ¹, Donghee Lee², Yunfan Kong ³, Mitchell Kuss ⁴, Ying Huang ⁵, Taesung Kim ⁶, Soonkyu Chung ⁷, Andrew T Dudley ⁸, Seung-Hyun Ro⁶, Bin Duan⁹

¹College of Biological Science and Medical Engineering, Donghua University, Shanghai 201620, China.; Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.; Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.

²Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.; Department of Surgery, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.

³Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.; Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.; Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.

⁴Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.; Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.

⁵Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.

⁶Department of Biochemistry and the Redox Biology Center, University of Nebraska-Lincoln, Lincoln, Nebraska 68588, USA.

⁷Department of Nutrition, University of Massachusetts Amherst, Amherst, Massachusetts 01003, USA.

⁸Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.; Department of Genetics, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.

⁹Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.; Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.; Department of Surgery, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.; Department of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska 68588, USA.

CORRESPONDING AUTHORS : Seung-Hyun Ro, Bin Duan

Abstract

Porous alginate hydrogels possess many advantages as cell carriers. However, current pore generation methods require either complex or harsh fabrication processes, toxic components, or extra purification steps, limiting the feasibility and affecting the cellular survival and function. In this study, a simple and cell-friendly approach to generate highly porous cell-laden alginate hydrogels based on two-phase aqueous emulsions is reported. The pre-gel solutions, which contain two immiscible aqueous phases of alginate and caseinate, are crosslinked by calcium ions. The porous structure of the hydrogel construct is formed by subsequently removing the caseinate phase from the ion-crosslinked alginate hydrogel. Those porous alginate hydrogels possess heterogeneous pores around 100 μm and interconnected paths. Human white adipose progenitors (WAPs) encapsulated in these hydrogels self-organize into spheroids and show enhanced viability, proliferation, and adipogenic differentiation, compared to non-porous constructs. As a proof of concept, this porous alginate hydrogel platform is employed to prepare core-shell spheres for coculture of WAPs and colon cancer cells, with WAP clusters distributed around cancer cell aggregates, to investigate cellular crosstalk. This efficacious approach is believed to provide a robust and versatile platform for engineering porous-structured alginate hydrogels for applications as cell carriers and in disease modeling.

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형식Research article
게재일2023년 08월 (BRIC 등록일 2024-01-15)
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