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Small Methods, Dec 31 2023, | https://doi.org/10.1002/smtd.202301428 Extracorporeal Blood Treatment Using Functional Magnetic Nanoclusters Mitigates Organ Dysfunction of Sepsis in Swine

Sung Jin Park ¹, Inwon Park ², Suhyun Kim ¹, Min Kyu Kim ¹, Seonghye Kim², Hwain Jeong ², Dongsung Kim ², Seung Woo Cho ¹, Tae-Eun Park ¹, Aleksey Ni ³, Hankwon Lim ³, Jinmyoung Joo ^1,4, Jae Hyuk Lee ², Joo H. Kang ¹

¹Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919, Republic of Korea.

²Department of Emergency Medicine, Seoul National University Bundang Hospital, Seongnam-si, Gyeonggi-do, 44919, Republic of Korea.

³School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919, Republic of Korea.

⁴Center for Genomic Integrity, Institute for Basic Science, Ulsan, 44919, Republic of Korea.

S.J.P, I.P., S.K., contributed equally to this work.

CORRESPONDING AUTHORS : Jinmyoung Joo, Jae Hyuk Lee, Joo H Kang

Abstract

Mitigating sepsis-induced severe organ dysfunction with magnetic nanoparticles has shown remarkable advances in extracorporeal blood treatment. Nevertheless, treating large septic animals remains challenging due to insufficient magnetic separation at rapid blood flow rates (>6 L h ^-1 ) and limited incubation time in an extracorporeal circuit. Herein, superparamagnetic nanoclusters (SPNCs) coated with red blood cell (RBC) membranes are developed, which promptly capture and magnetically separate a wide range of pathogens at high blood flow rates in a swine sepsis model. The SPNCs exhibited an ultranarrow size distribution of clustered iron oxide nanocrystals and exceptionally high saturation magnetization (≈ 90 emu g ^-1 ) close to that of bulk magnetite. It is also revealed that CD47 on the RBCs allows the RBC-SPNCs to remain at a consistent concentration in the blood by evading innate immunity. The uniform size distribution of the RBC-SPNCs greatly enhances their effectiveness in eradicating various pathogenic materials in extracorporeal blood. The use of RBC-SPNCs for extracorporeal treatment of swine infected with multidrug-resistant E. coli is validated and found that severe bacteremic sepsis-induced organ dysfunction is significantly mitigated after 12 h. The findings highlight the potential application of RBC-SPNCs for extracorporeal therapy of severe sepsis in large animal models and potentially humans.

논문정보

형식Research article
게재일2023년 12월 (BRIC 등록일 2024-01-03)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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