한빛사논문
- 조회499
Geonhee Han a,b, Hyosuk Kim b, Hochung Jang b,c, Eun Sun Kim d, Sun Hwa Kim a,b, Yoosoo Yang b,c
aKU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, 02841, Republic of Korea
bMedicinal Materials Research Center, Biomedical Research Division, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of Korea
cDivision of Bio-Medical Science and Technology, KIST School, University of Science and Technology, Seoul, 02792, Republic of Korea
dDepartment of Internal Medicine, Korea University College of Medicine, Seoul, 02841, Republic of Korea
Corresponding authors: Sun Hwa Kim, Yoosoo Yang
Abstract
Oral administration facilitates the direct delivery of drugs to lesions within the small intestine and colon, making it an ideal approach for treating patients with inflammatory bowel disease. However, multiple physical barriers impede the delivery of oral RNA drugs through the gastrointestinal tract. Herein, we developed a novel oral siRNA delivery system that protects nucleic acids in extreme environments by employing exosomes derived from milk to encapsulate tumor necrosis factor-alpha (TNF-α) siRNA completely. The remarkable structural stability of milk-derived exosomes (M-Exos), as opposed to those from HEK293T cells, makes them exceptional siRNA carriers. Results demonstrate that milk exosomes loaded with TNF-α siRNA (M-Exo/siR) can effectively inhibit the expression of TNF-α-related inflammatory cytokines. Moreover, given that milk exosomes are composed of unique lipids with high bioavailability, orally administered M-Exo/siR effectively reach colonic tissues, leading to decreased TNF-α expression and successful alleviation of colitis symptoms in a dextran sulfate sodium-induced inflammatory bowel disease murine model. Hence, milk-derived exosomes carrying TNF-α siRNA can be effectively employed to treat inflammatory bowel disease. Indeed, using exosomes naturally derived from milk may shift the current paradigm of oral gene delivery, including siRNA.
논문정보
- Research article
- 2023년 12월 (BRIC 등록일 2023-12-28)
- 국내 연구진
- 바이오·의료융합 > 바이오·의료융합 기타
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