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Cell Syst., Dec 20 2023, 14 (12) S2405-4712(23)00301-0 | https://doi.org/10.1016/j.cels.2023.10.009
Advances in ligand-specific biosensing for structurally similar molecules
Chenggang Xi 1, Jinjin Diao 1, Tae Seok Moon 1,2
1Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, St. Louis, MO, USA
2Division of Biology and Biomedical Sciences, Washington University in St. Louis, St. Louis, MO, USA
Corresponding author : Tae Seok Moon
Abstract
The specificity of biological systems makes it possible to develop biosensors targeting specific metabolites, toxins, and pollutants in complex medical or environmental samples without interference from structurally similar compounds. For the last two decades, great efforts have been devoted to creating proteins or nucleic acids with novel properties through synthetic biology strategies. Beyond augmenting biocatalytic activity, expanding target substrate scopes, and enhancing enzymes' enantioselectivity and stability, an increasing research area is the enhancement of molecular specificity for genetically encoded biosensors. Here, we summarize recent advances in the development of highly specific biosensor systems and their essential applications. First, we describe the rational design principles required to create libraries containing potential mutants with less promiscuity or better specificity. Next, we review the emerging high-throughput screening techniques to engineer biosensing specificity for the desired target. Finally, we examine the computer-aided evaluation and prediction methods to facilitate the construction of ligand-specific biosensors.
논문정보
- Review Paper
- 2023년 12월 (BRIC 등록일 2023-12-26)
- 국외 연구진
- 생명과학 > 생물공학
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