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중앙대학교, 나노메디신

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Adv. Healthc. Mater., Dec 20 2023, | https://doi.org/10.1002/adhm.202302313 Dielectrophoretic Capture of Cancer-Derived Small Extracellular Vesicles-Bound Janus Nanoparticles via Lectin-Glycan Interaction

Yonghyun Choi^a,b, Kubra Akyildiz^c, Jihyun Seong^d, Yangwoo Lee^c, Eunseo Jeong^a,b, Jin-seok Park^e, Don Haeng Lee^e, Kyobum Kim^d,*, Hyung-Jun Koo^c,*, Jonghoon Choi^a,b,*

^aSchool of Integrative Engineering, Chung-Ang University, Seoul06974, Republic of Korea

^bFeynman Institute of Technology, Nanomedicine Corporation, Seoul 06974, Republic of Korea

^cDepartment of Chemical and Biomolecular Engineering, Seoul National University of Science and Technology, Seoul, 01811, Republic of Korea

^dDepartment of Chemical & Biochemical Engineering, Dongguk University, Seoul, 04620, Republic of Korea

^eDepartment of Internal Medicine, Inha University School of Medicine, Incheon, 22212, Republic of Korea

^*Corresponding authors: Kyobum Kim, Hyung-Jun Koo, Jonghoon Choi

Abstract

Glycosylation is closely related to cell metabolism and disease progression. Particularly, cancer progression increases the glycan levels of cancer cells and tissues. This up-regulation of glycosylation in cancer cells may provide a basis for the development of new biomarkers for targeting and diagnosing specific cancers. Here, we developed a detection technology for pancreatic cancer cell-derived small extracellular vesicles (PC-sEVs) based on lectin-glycan interactions. Afterward, lectins with a strong and specific affinity for sialic acid were conjugated on bifunctional nanoparticles (i.e., Janus nanoparticles) to induce interactions with PC-sEVs in a dielectrophoretic (DEP) system. PC-sEVs from cell lines and clinical samples were selectively bound on the lectin-conjugated Janus nanoparticles (Lectin-JNPs) with comparable affinity to that of conventionally used carbohydrate antigen 19-9 (CA19-9) antibodies. Furthermore, sEVs-bound Lectin-JNPs (sEVs-Lec-JNPs) were manipulated between two electrodes to which an AC signal was applied for DEP capture. Additionally, the proposed DEP system can be used to trap the sEVs -Lec-JNPs on the electrodes. Our findings, which were confirmed by Lectin-JNPs using the proposed DEP system, followed by target gene analysis, provide a basis for the development of a new early diagnosis marker based on the glycan properties of PC- sEVs. In turn, these novel detection methods could overcome the shortcomings of commercially available pancreatic cancer detection techniques. This article is protected by copyright. All rights reserved.

논문정보

형식Research article
게재일2023년 12월 (BRIC 등록일 2023-12-22)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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