한빛사논문
- 조회434
Sijin Park1†, Dong Yeon Nam2†, Hee‑Jae Jeon3†, Jae Hoon Han2†, Dawon Jang1, Juil Hwang4, Yeong‑Seo Park3, Young‑Geun Han4, Young Bin Choy2,5,6* and Dong Yun Lee1,7,8*
1Department of Bioengineering, College of Engineering, and BK FOUR Biopharmaceutical Innovation Leader for Education and Research Group, Hanyang University, 222 Wangsimni‑Ro Seongdong‑Gu, Seoul 04763, Republic of Korea.
2College of Engineering, Interdisciplinary Program in Bioengineering, Seoul National University, 1 Gwanak‑Ro Gwanak‑Gu, Seoul 08826, Republic of Korea.
3Department of Mechanical and Biomedical Engineering, Kangwon National University, 1 Gangwondaehak‑Gil, Chuncheon 24341, Republic of Korea.
4Department of Physics, College of Natural Sciences, Hanyang University, 222 Wangsimni‑Ro Seongdong‑Gu, Seoul 04763, Republic of Korea.
5Department of Biomedical Engineering, Seoul National University College of Medicine, 101 Daehak‑Ro Jongno‑Gu, Seoul 03080, Republic of Korea.
6Institute of Medical & Biological Engineering, Medical Research Center, Seoul National University, 101 Daehak‑Ro Jongno‑Gu, Seoul 03080, Republic of Korea.
7Institute of Nano Science and Technology (INST) and Institute for Bioengineering and Biopharmaceutical Research (IBBR), Hanyang University, 222 Wangsimni‑Ro Seongdong‑Gu, Seoul 04763, Republic of Korea.
8Elixir Pharmatech Inc, 222 Wangsimni‑Ro Seongdong‑Gu, Seoul 04763, Republic of Korea.
Sijin Park, Dong Yeon Nam, Hee-Jae Jeon and Jae Hoon Han contributed equally to this work.
Corresponding authors : Correspondence to Young Bin Choy or Dong Yun Lee.
Abstract
Background
Noninvasive monitoring of tear glucose levels can be convenient for patients to manage their diabetes mellitus. However, there are issues with monitoring tear glucose levels, such as the invasiveness of some methods, the miniaturization, inaccuracy, or the high cost of wearable devices. To overcome the issues, we newly designed a sucking disk-type (SD) strip biosensor that can quickly suck tear fluid and contains cerium oxide nanoparticle (CNP) that causes a unique color change according to the glucose level of the tear without complicated electronic components.
Methods
The SD strip biosensor composed of three distinct parts (tip, channel, and reaction chamber) was designed to contain the sensing paper, onto which tear fluid can be collected and delivered. The sensing paper treated with CNP/APTS (aminopropyltriethoxysilane) /GOx (glucose oxidase) was characterized. Then we carried out the reliability of the SD strip biosensor in the diabetic rabbit animals. We quantitatively analyzed the color values of the SD strip biosensor through the colorimetric analysis algorithm.
Results
We contacted the inferior palpebral conjunctiva (IPC) of a diabetic rabbit eye using an SD strip biosensor to collect tears without eye irritation and successfully verified the performance and quantitative efficacy of the sensor. An image processing algorithm that can optimize measurement accuracy is developed for accurate color change measurement of SD strip biosensors. The validation tests show a good correlation between glucose concentrations measured in the tear and blood.
Conclusion
Our findings demonstrate that the CNP-embedded SD strip biosensor and the associated image processing can simply monitor tear glucose to manage diabetes mellitus.
논문정보
- Research article
- 2023년 12월 (BRIC 등록일 2023-12-19)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
관련 보도자료
- Bio통신원
- 2024. 01. 08
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