신경식 (바이오소닉스)

한빛사논문

조회236

신경식Keyong-Sik Shin

바이오소닉스

Biosens. Bioelectron., Dec 05 2023, 247 S0956-5663(23)00840-0 | https://doi.org/10.1016/j.bios.2023.115898 Implementation of an ultra-sensitive microwell-based electrochemical sensor for the detection of Alzheimer's disease

Soumi Roy ^a,b, Sarang Kang ^c,d, Kyu Yeong Choi^c,e, Kun Ho Lee ^c,f,g, Keyong-Sik Shin ^h, Ji Yoon Kang ^a,b

^aBrain Science Institute, Biomedical Engineering, Korea Institute of Science and Technology, KIST School, Seoul, 02792, Republic of Korea

^bDepartment of Biomedical Engineering, University of Science and Technology, Daejeon, Republic of Korea

^cGwangju Alzheimer's Disease and Related Dementia Cohort Research Center, Chosun University, Gwangju, 61452, Republic of Korea

^dBK21 FOUR Educational Research Group for Age-Associated Disorder Control Technology, Chosun University, Gwangju, 61452, Republic of Korea

^eKolab Inc., Gwangju, 61436, Republic of Korea

^fDepartment of Biomedical Science, Chosun University, Gwangju, 61452, Republic of Korea

^gKorea Brain Research Institute, Daegu, 41062, Republic of Korea

^hBioxonics Co. Ltd., Seoul, 02792, Republic of Korea

Corresponding authors: Keyong-Sik Shin, Ji Yoon Kang

Abstract

Alzheimer's Disease (AD) is one of the most common neurodegenerative disorders in elderly people. It is diagnosed by detecting amyloid beta (Aβ) protein in cerebrospinal fluid (CSF) obtained by lumbar puncture or through expensive positron emission tomography (PET) imaging. Although blood-based diagnosis of AD offers a less invasive and cost-effective alternative, the quantification of Aβ is technically challenging due to its low abundance in peripheral blood. To address this, we developed a compact yet highly sensitive microwell-based electrochemical sensor with a densely packed microelectrode array (20 by 20) for enhancing sensitivity. Employing microwells on the working and counter electrodes minimized the leakage current from the metallic conductors into the assay medium, refining the signal fidelity. We achieved a detection limit <10 fg/mL for Aβ by elevating the signal-to-noise ratio, thus capable of AD biomarker quantification. Moreover, the microwell structure maintained the performance irrespective of variations in bead number, indicative of the sensor's robustness. The sensor's efficacy was validated through the analysis of Aβ concentrations in plasma samples from 96 subjects, revealing a significant distinction between AD patients and healthy controls with an area under the receiver operating characteristic curve (AUC) of 0.85. Consequently, our novel microwell-based electrochemical biosensor represents a highly sensitive platform for detecting scant blood-based biomarkers, including Aβ, offering substantial potential for advancing AD diagnostics.

논문정보

형식Research article
게재일2023년 12월 (BRIC 등록일 2023-12-18)
연구진국내 연구진
분야

댓글 작성 로그인

CV 열람하기

연락처 보기