상위피인용논문
- 조회463
Sung Ho Yoon 1, Seong Keun Kim 1 and Jihyun F. Kim 1,2,*
1Industrial Biotechnology and Bioenergy Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 111 Gwahangno, Yuseong, Daejeon 305-806, Republic of Korea
2Functional Genomics Program, School of Science, University of Science and Technology, Yuseong, Daejeon 305-333, Republic of Korea
*Corresponding author: correspondence to Jihyun F. Kim
Abstract
Extracellular production of heterologous proteins using the Escherichia coli cell factory offers several advantages over intracellular production and mammalian culture. Properly folded proteins can be rapidly accumulated in the culture media, and downstream processes for isolation and purification can be much simplified. Efforts to enhance the secretory production of target proteins can be largely classified as selection and modification of the signal peptide, co-expression of proteins to assist translocation and folding, improvement of periplasmic release, and protection of target proteins from degradation and contamination. Here, we review recent patents on the secretory production of recombinant proteins in E. coli.
논문정보
- Review Paper
- 2010년 01월 (BRIC 등록일 2023-12-13)
- 국내 연구진
- 바이오·의료융합 > 바이오소재
- 120회 이상 인용된 논문
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