박윤경 (조선대학교)

한빛사논문

조회370

조선대학교

CV File 229 kb

Int. J. Antimicrob. Agents, Dec 08 2023, S0924-8579(23)00343-6 | https://doi.org/10.1016/j.ijantimicag.2023.107054 Efficacy of the bee-venom antimicrobial peptide Osmin against sensitive and carbapenem-resistant Klebsiella pneumoniae strains

Eunyeong Jeon ^a, Min Kyung Kim ^a, Yoonkyung Park ^a,b

^aDepartment of Biomedical Sciences, Chosun University, Gwangju, 61452, Republic of Korea

^bResearch Center for Proteineous Materials (RCPM), Chosun University, Gwangju, 61452, Republic of Korea

Corresponding author: Yoonkyung Park

Abstract

The emergence of multidrug-resistant (MDR) Klebsiella pneumoniae strains causes severe problems in the treatment of bacterial infections owing to limited treatment options. Especially, carbapenem-resistant K. pneumoniae (CRKP) is rapidly spreading worldwide and is emerging as a new cause of drug-resistant healthcare-associated infections. CRKP also has been announced by the Centers for Disease Control and Prevention (CDC) and the World Health Organization (WHO) as one of the most pressing antibiotic resistance threats. Antimicrobial peptides (AMPs) are drawing considerable attention as ideal antibiotic alternative candidates to combat MDR bacterial infections. In a previous report, Osmin is composed of 17 amino acids and is isolated from solitary bee (Osmia rufa) venom. Herein, we evaluated the potential of Osmin to be used against drug-resistant K. pneumoniae as an alternative to conventional antibiotics. Osmin exhibited significant antimicrobial and anti-biofilm activity and lower toxicity than melittin, a well-known bee venom peptide. Additionally, we confirmed that it possesses a bactericidal mechanism that rapidly destroys bacterial membranes. Osmin was relatively more stable than melittin under the influence of various environmental factors and unlike conventional antibiotics, it exhibited a low bacterial resistance risk. During in vivo tests, Osmin reduced bacterial growth and the expression of pro-inflammatory cytokines and fibrosis-related genes in mice with CRKP-induced sepsis. Overall, our results indicate a high potential for Osmin to be used as a valuable therapeutic agent against drug-resistant K. pneumoniae infections.

논문정보

형식Research article
게재일2023년 12월 (BRIC 등록일 2023-12-12)
연구진국내 연구진
분야 생명과학 > 면역학

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