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Bioresour. Technol., Dec 08 2023, 393 S0960-8524(23)01586-9 | https://doi.org/10.1016/j.biortech.2023.130158 Bioconversion of citrus waste into mucic acid by xylose-fermenting Saccharomyces cerevisiae

Deokyeol Jeong ^a, Sujeong Park^b, Grace Evelina^b, Suhyeung Kim ^b, Heeyoung Park ^b, Je Min Lee^c, Sun-Ki Kim ^d, In Jung Kim ^e, Eun Joong Oh ^a, Soo Rin Kim ^b,f

^aDepartment of Food Science, Purdue University, West Lafayette, IN 47907, United State

^bSchool of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea

^cDepartment of Horticultural Science, Kyungpook National University, Daegu 41566, Republic of Korea

^dDepartment of Food Science and Technology, Chung-Ang University, Anseong, Gyeonggi 17546, Republic of Korea

^eDepartment of Food Science & Technology, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea

^fResearch Institute of Tailored Food Technology, Kyungpook National University, Daegu 41566, Republic of Korea

Corresponding authors : Eun Joong Oh, Soo Rin Kim

Abstract

Mucic acid holds promise as a platform chemical for bio-based nylon synthesis; however, its biological production encounters challenges including low yield and productivity. In this study, an efficient and high-yield method for mucic acid production was developed by employing genetically engineered Saccharomyces cerevisiae expressing the NAD ⁺-dependent uronate dehydrogenase (udh) gene. To overcome the NAD ⁺ dependency for the conversion of pectin to mucic acid, xylose was utilized as a co-substrate. Through optimization of the udh expression system, the engineered strain achieved a notable output, producing 20 g/L mucic acid with a highest reported productivity of 0.83 g/L-h and a theoretical yield of 0.18 g/g when processing pectin-containing citrus peel waste. These results suggest promising industrial applications for the biological production of mucic acid. Additionally, there is potential to establish a viable bioprocess by harnessing pectin-rich fruit waste alongside xylose-rich cellulosic biomass as raw materials.

논문정보

형식Research article
게재일2023년 12월 (BRIC 등록일 2023-12-12)
연구진국내(교신)+국외 연구진
분야 생명과학 > 식품학

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