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Int. J. Oral Sci., Dec 07 2023, 15 (1) 55 | https://doi.org/10.1038/s41368-023-00257-w Adult dental epithelial stem cell-derived organoids deposit hydroxylapatite biomineral

Hyun-Yi Kim¹, Victoria Cooley^2,3, Eun-Jung Kim⁴, Shujin Li⁴, Jong-Min Lee⁴, Dina Sheyfer³, Wenjun Liu³, Ophir D. Klein^5,6, Derk Joester² and Han-Sung Jung⁴

¹NGeneS Inc., Ansan-si, Gyeonggi-do, Korea;

²Department of Materials Science and Engineering, Northwestern University, Evanston, IL, USA;

³X-ray Science Division, Advanced Photon Source, Argonne National Laboratory, Lemont, IL, USA;

⁴Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 FOUR Project, Yonsei University College of Dentistry, Seoul, Korea;

⁵Department of Orofacial Sciences and Program in Craniofacial Biology, University of California, San Francisco, CA, USA and

⁶Department of Pediatrics, Cedars-Sinai Guerin Children’s, Los Angeles, CA, USA

Correspondence: Han-Sung Jung

Abstract

Ameloblasts are specialized cells derived from the dental epithelium that produce enamel, a hierarchically structured tissue comprised of highly elongated hydroxylapatite (OHAp) crystallites. The unique function of the epithelial cells synthesizing crystallites and assembling them in a mechanically robust structure is not fully elucidated yet, partly due to limitations with in vitro experimental models. Herein, we demonstrate the ability to generate mineralizing dental epithelial organoids (DEOs) from adult dental epithelial stem cells (aDESCs) isolated from mouse incisor tissues. DEOs expressed ameloblast markers, could be maintained for more than five months (11 passages) in vitro in media containing modulators of Wnt, Egf, Bmp, Fgf and Notch signaling pathways, and were amenable to cryostorage. When transplanted underneath murine kidney capsules, organoids produced OHAp crystallites similar in composition, size, and shape to mineralized dental tissues, including some enamel-like elongated crystals. DEOs are thus a powerful in vitro model to study mineralization process by dental epithelium, which can pave the way to understanding amelogenesis and developing regenerative therapy of enamel.

논문정보

형식Research article
게재일2023년 12월 (BRIC 등록일 2023-12-11)
연구진국내(교신)+국외 연구진
분야 생명과학 > 세포생물학

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