김동립 (한양대학교) | 한빛사논문 > 한빛사

한빛사논문

조회385

한양대학교

ACS Nano, Dec 07 2023, | https://doi.org/10.1021/acsnano.3c07527 Transparent Intracellular Sensing Platform with Si Needles for Simultaneous Live Imaging

Woohyun Park ¹, Eun Mi Kim ², Yale Jeon³, Junsang Lee ^3,4, Jonghun Yi ³, Jinheon Jeong ⁴, Bongjoong Kim ^1,5, Byeong Guk Jeong ^3,4, Dong Rip Kim ³, Hyunjoon Kong ^2,6, Chi Hwan Lee^1,4,7

¹School of Mechanical Engineering, Purdue University, West Lafayette, Indiana 47907, United States.

²Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.

³School of Mechanical Engineering, Hanyang University, Seoul 04763, Republic of Korea.

⁴Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907, United States.

⁵Department of Mechanical and System Design Engineering, Hongik University, Seoul 04066, Republic of Korea.

⁶Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.

⁷Department of Materials Engineering, Purdue University, West Lafayette, Indiana 47907, United States.

W.P., E.M.K., Y.J., and J.L. contributed equally to this work.

Corresponding Authors : Dong Rip Kim, Hyunjoon Kong, Chi Hwan Lee

Abstract

Vertically ordered Si needles are of particular interest for long-term intracellular recording owing to their capacity to infiltrate living cells with negligible damage and minimal toxicity. Such intracellular recordings could greatly benefit from simultaneous live cell imaging without disrupting their culture, contributing to an in-depth understanding of cellular function and activity. However, the use of standard live imaging techniques, such as inverted and confocal microscopy, is currently impeded by the opacity of Si wafers, typically employed for fabricating vertical Si needles. Here, we introduce a transparent intracellular sensing platform that combines vertical Si needles with a percolated network of Au-Ag nanowires on a transparent elastomeric substrate. This sensing platform meets all prerequisites for simultaneous intracellular recording and imaging, including electrochemical impedance, optical transparency, mechanical compliance, and cell viability. Proof-of-concept demonstrations of this sensing platform include monitoring electrical potentials in cardiomyocyte cells and in three-dimensionally engineered cardiovascular tissue, all while conducting live imaging with inverted and confocal microscopes. This sensing platform holds wide-ranging potential applications for intracellular research across various disciplines such as neuroscience, cardiology, muscle physiology, and drug screening.

논문정보

형식Research article
게재일2023년 12월 (BRIC 등록일 2023-12-11)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

댓글 작성 로그인

CV 열람하기

연락처 보기