김찬홍 (Chinese Academy of Sciences)

한빛사논문

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Chinese Academy of Sciences

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Plant Commun., December 03, 2023 | https://doi.org/10.1016/j.xplc.2023.100775 Alternaria TeA toxin activates a chloroplast retrograde signaling pathway to facilitate JA-dependent pathogenicity

Jiale Shi ¹, He Wang ¹, Mengping Li ^2,a, Liru Mi ¹, Yazhi Gao ¹, Sheng Qiang¹, Yu Zhang ^{1 3}, DanChen ¹, Xinbin Dai ³, Hongyu Ma ⁴, Huan Lu 1, Chanhong Kim ^2,*, Shiguo Chen ^1,*

¹Weed Research Laboratory, Nanjing Agricultural University, Nanjing 210095, China
²Shanghai Center for Plant Stress Biology, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China
³Bioinformatics and Computational Biology Laboratory, Noble Research Institute, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA
⁴College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
^aCurrent address: Department of Botany and Plant Biology, University of Geneva, Geneva, Switzerland

^*Corresponding authors: correspondence to Chanhong Kim or Shiguo Chen

Abstract

The chloroplast is a critical battleground in the arms race between plants and pathogens. Among microbe-secreted mycotoxins, the tenuazonic acid (TeA), produced by the genus Alternaria and other phytopathogenic fungi, inhibits photosynthesis, leading to a burst of photosynthetic singlet oxygen (¹O₂) implicated in damage and chloroplast-to-nucleus retrograde signaling. Despite the potent adversity of Alternaria pathogens on crop production, the molecular mechanism of TeA-caused pathogenicity and cognate plant defense responses remain fragmented. We now reveal that A. alternata induces necrotrophic foliar lesions by harnessing an EXECUTER1 (EX1)/EX2-mediated chloroplast-to-nucleus retrograde signaling (RS), activated by TeA toxin-derived photosynthetic ¹O₂ in Arabidopsis thaliana. Mutation of ¹O₂-sensitive EX1-W643 or complete deletion of the SOS (Singlet Oxygen Sensor) domain of EX1 compromises the expression of ¹O₂-responsive nuclear genes and foliar lesions. Remarkably, we further noticed that the TeA toxin rapidly induces nuclear genes implicated in jasmonic acid (JA) synthesis and signaling, for which EX1-mediated RS appeared to be critical in establishing a signaling cascade from ¹O₂ to JA. Our present study sheds new light on A. alternata-caused foliar pathogenicity, where EX1-dependent ¹O₂ signaling induces JA-dependent foliar cell death.

논문정보

형식Research article
게재일2023년 12월 (BRIC 등록일 2024-01-16)
연구진국외 연구진
분야 생명과학 > 식물학

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