한빛사논문
- 조회561
Muhammad Junaid1, Han Kyoung Choe2, Kunio Kondoh3, Eun Jeong Lee 4 & Su Bin Lim 1
1Department of Biochemistry & Molecular Biology, Ajou University School of Medicine, Suwon, 16499, Korea.
2Department of Brain Sciences, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, 42988, Korea.
3Division of Endocrinology and Metabolism, Department of Homeostatic Regulation, National Institute for Physiological Sciences, National Institutes of Natural Sciences, Okazaki, Aichi, 444-8585, Japan.
4Department of Brain Science, Ajou University School of Medicine, Suwon, 16499, Korea.
Corresponding authors : Correspondence to Eun Jeong Lee or Su Bin Lim.
Abstract
Despite the importance of hypothalamic neurocircuits in regulating homeostatic and survival-related behaviors, our understanding of the intrinsic molecular identities of neural components involved in these complex multi-synaptic interactions remains limited. In this study, we constructed a Cre recombinase-dependent pseudorabies virus (PRVs) capable of crossing synapses, coupled with transcriptome analysis of single upstream neurons post-infection. By utilizing this retrograde nuclear Connect-seq (nuConnect-seq) approach, we generated a single nuclei RNA-seq (snRNA-seq) dataset of 1,533 cells derived from the hypothalamus of CRH-IRES-Cre (CRH-Cre) mice. To ensure the technical validity of our nuConnect-seq dataset, we employed a label transfer technique against an integrated reference dataset of postnatal mouse hypothalamus comprising 152,524 QC-passed cells. The uniqueness of our approach lies in the integration of diverse datasets for validation, providing a more nuanced diversity of hypothalamic cell types. The presented validated dataset may deepen our understanding of hypothalamic neurocircuits and underscore the essential role of comprehensive integrated transcriptomic data for technical validity.
논문정보
- Research article
- 2023년 12월 (BRIC 등록일 2023-12-05)
- 국내(교신)+국외 연구진
- 의약학 > 유전 및 유전체의학
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