Xuan Ai Le (가천대학교) | 한빛사논문 > 한빛사

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Xuan Ai LeXuan Ai Le

가천대학교

Biosens. Bioelectron., Nov 27 2023, 246 S0956-5663(23)00824-2 | https://doi.org/10.1016/j.bios.2023.115882 Active site engineering of Zn-doped mesoporous ceria toward highly efficient organophosphorus hydrolase-mimicking nanozyme

Junsang Lee ^a,1, Xuan Ai Le ^b,1, Hoje Chun^c,1, Trung Hieu Vu ^b, Daeeun Choi ^a, Byungchan Han ^c, Moon Il Kim ^b, Jinwoo Lee ^a

^aDepartment of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea

^bDepartment of BioNano Technology, Gachon University, Seongnam, Gyeonggi, 13120, Republic of Korea

^cDepartment of Chemical and Biomolecular Engineering, Yonsei University, Seoul, 03722, Republic of Korea

¹These authors contributed equally to this work.

Corresponding authors: Byungchan Han, Moon Il Kim, Jinwoo Lee

Abstract

Hydrolase-mimicking nanozymes have received increasing attention in recent years, but the effective rational design and development of these materials has not been realized, as they are not at present considered a critical research target. Herein, we report that Zn-doped mesoporous ceria (Zn-m-ceria) engineered to have an abundance of two different active sites with different functions-one that allows both co-adsorption binding of organophosphate (OP) and water and another that serves as a general base-has significant organophosphorus hydrolase (OPH)-like catalytic activity. Specifically, Zn-m-ceria exhibits a catalytic efficiency over 75- and 25-fold higher than those of m-ceria and natural OPH, respectively. First-principles calculations reveal the importance of Zn for the OPH-mimicking activity of the material, promoting substrate adsorption and proton-binding. The OPH-like Zn-m-ceria catalyst is successfully applied to detect a model OP, methyl paraoxon, in spiked tap water samples with excellent sensitivity, stability, and detection precision. We expect that these findings will promote research based on the rational engineering of the active site of nanozymes and efficient strategies for obtaining a diverse range of catalysts that mimic natural enzymes, and hence the utilization in real-world applications of enzyme-mimicking catalysts with properties superior to their natural analogs should follow.

논문정보

형식Research article
게재일2023년 11월 (BRIC 등록일 2023-12-04)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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