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Chinese Academy of Sciences

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Plant Cell, Dec 02 2023, koad300 | https://doi.org/10.1093/plcell/koad300 The m6A reader ECT1 drives mRNA sequestration to dampen salicylic acid-dependent stress responses in Arabidopsis

Keun Pyo Lee ¹, Kaiwei Liu ^1,2, Eun Yu Kim ³, Laura Medina-Puche ¹, Haihong Dong¹, Minghui Di ^1,2, Rahul Mohan Singh ¹, Mengping Li¹, Shan Qi ^1,2, Zhuoling Meng ^1,2, Jungnam Cho ^3,4, Heng Zhang ¹, Rosa Lozano-Duran¹, Chanhong Kim ¹

¹Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences (CEMPS), Chinese Academy of Sciences, Shanghai 200032, China.

²University of the Chinese Academy of Sciences, Beijing 100049, China.

³National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences (CEMPS), Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China.

⁴CAS-JIC Centre of Excellence for Plant and Microbial Science, Chinese Academy of Sciences, Shanghai, 200032, China.

Keun Pyo Lee, Kaiwei Liu and Eun Yu Kim contributed equally to this work.

Corresponding author: Chanhong Kim

Abstract

N 6-methyladenosine (m6A) is a common epitranscriptional mRNA modification in eukaryotes. Thirteen putative m6A readers, mostly annotated as EVOLUTIONARILY CONSERVED C-TERMINAL REGION (ECT) proteins, have been identified in Arabidopsis (Arabidopsis thaliana), but few have been characterized. Here, we show that the Arabidopsis m6A reader ECT1 modulates salicylic acid (SA)-mediated plant stress responses. ECT1 undergoes liquid-liquid phase separation in vitro, and its N-terminal prion-like domain is critical for forming in vivo cytosolic biomolecular condensates in response to SA or bacterial pathogens. Fluorescence-activated particle sorting coupled with quantitative PCR analyses unveiled that ECT1 sequesters SA-induced m6A modification-prone mRNAs through its conserved aromatic cage to facilitate their decay in cytosolic condensates, thereby dampening SA-mediated stress responses. Consistent with this finding, ECT1 overexpression promotes bacterial multiplication in plants. Collectively, our findings unequivocally link ECT1-associated cytosolic condensates to SA-dependent plant stress responses, advancing the current understanding of m6A readers and the SA signaling network.

논문정보

형식Research article
게재일2023년 12월 (BRIC 등록일 2023-12-04)
연구진국외 연구진
분야 생명과학 > 식물학

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