한빛사논문
- 조회360
Kyung Ho Kim1.2, Sung Eun Seo1, Jinyeong Kim1, Seon Joo Park1, Jai Eun An1, Chan Jae Shin3, Choong-Min Ryu1, Sung Woon Lee4, Ho Chul Nam4, Tae Ho Yoon4, Jong Cheol Shin4, Yu Kyung Kim5, Hanseul Oh6, Jung Joo Hong7,8, Brian N. Kim9, Kyoung G. Lee10, Hyun Seok Song11, Sang Hun Lee12*, and Oh Seok Kwon1,3*
1Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141 Republic of Korea
2Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141 Republic of Korea
3SKKU Advanced Institute of Nanotechnology (SAINT), Department of Nano Engineering, Sungkyunkwan University (SKKU), Suwon, 16419 Republic of Korea
4RevoSketch Inc., 506, 1662, YuSeong-daero, YuSeong-gu, Daejeon, 34054 Republic of Korea
5Department of Clinical Pathology, School of Medicine, Kyungpook National University, Daegu, 41944 Republic of Korea
6College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungcheongbuk, 28644 Republic of Korea
7National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Chungcheongbuk, 28116 Republic of Korea
8Korea Research Institute of Bioscience and Biotechnology (KRIBB) School of Bioscience, Korea University of Science & Technology (UST), Daejeon, 34113 Republic of Korea
9Department of Electrical and Computer Engineering, University of Central Florida, Orlando, FL, USA
10Center for Nano Bio Development National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141 South Korea
11Sensor System Research Center, Korea Institute of Science and Technology, Seoul, 02792 Republic of Korea
12Department of Chemcial and Biological Engineering, Hanbat National University, Daejeon, 34158 Republic of Korea
K.H.K. and S.E.S. contributed equally to this work.
CORRESPONDING AUTHORS: Sang Hun Lee, Oh Seok Kwon
Abstract
A molecular diagnosis of the respiratory syncytial virus (RSV) without bulky and expensive instrumentation is of great importance for the early detection and prevention in a fast-spreading pandemic. However, the current representative diagnostic methods have the limitation of being time-consuming, cost, the processing time for polymerase chain reaction (PCR), and inaccurate for lateral flow assay (LFA), representatively. Herein, an integrated photonic digital PCR (dPCR) is developed with high-velocity photonic scanner for in situ fluorescence detection by introducing the N-heterocyclic carbene self-assembled monolayer-based Au film to prevent the quenching effect. The on-site rapid molecular diagnostic platform shows the driving of 40 cycles in under 8 min and fluorescence scanning in under 7 min, resulting in a total analysis time within 15 min. In particular, the technology clearly demonstrates the classification of SARS-CoV-2 patients and healthy controls (99% in sensitivity, 98.6% in specificity, and 96.4% in accuracy with RdRp gene), comparing with standard RT-qPCR. This platform can be utilized for prompt point-of-care molecular diagnostics in early diagnosis and large-scale prevention of next pandemic spreading for upcoming infectious diseases and for the distinction diagnosis with other RSV.
논문정보
- Research article
- 2023년 09월 (BRIC 등록일 2023-11-29)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 바이오·의료융합 기타
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