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New Phytol., Nov 28 2023, | https://doi.org/10.1111/nph.19430
Oomycete effector AVRblb2 targets cyclic nucleotide-gated channels through calcium sensors to suppress pattern-triggered immunity
Soeui Lee1,2, Hye-Young Lee1, Hui Jeong Kang1, Ye-Eun Seo1,2, Joo Hyun Lee1* and Doil Choi1,2*
1Plant Immunity Research Center, Plant Genomics and Breeding Institute, Seoul National University, Seoul, 08826, Korea;
2Horticultural Biotechnology, Department of Agriculture, Forestry, and Bioresources, College of Agriculture and Life Science, Seoul National University, Seoul, 08826, Korea
*These authors contributed equally to this work.
Authors for correspondence: Joo Hyun Lee, Doil Choi
Abstract
Transient and rapid increase in cytosolic Ca
2+ plays a crucial role in plant-pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI). Cyclic nucleotide-gated channels (CNGCs) have been implicated in mediating this Ca
2+ influx; however, their regulatory mechanisms remain poorly understood. Here, we have found that AVRblb2 requires the calmodulin (CaM) and calmodulin-like (CML) proteins as co-factors to interact with the NbCNGCs, resulting in the formation of AVRblb2-CaM/CML-NbCNGCs complex. Furthermore, CaM and CML are dissociated from NbCNGC18 during PTI response to increase Ca
2+ influx; however, Avrblb2 inhibits calcium channel activation by disrupting the release of CaM and CML from NbCNGC18. Following recognition of PAMP, NbCNGC18 forms active heteromeric channels with other NbCNGCs, which may give selectivity of CNGC complex against diverse signals for fine-tuning of cytosolic Ca
2+ level to mediate appropriate responses. Silencing of multiple NbCNGCs compromised the function of AVRblb2 on the pathogenicity of Phytophthora infestans, confirming that AVRblb2 contributes to pathogen virulence by targeting CNGCs. Our findings provide new insights into the regulation of CNGCs in PTI and the role of pathogen effectors in manipulating host cell physiology to promote infection.
논문정보
- Research article
- 2023년 11월 (BRIC 등록일 2023-12-01)
- 국내 연구진
- 생명과학 > 식량작물 및 원예작물
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