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New Phytol., Nov 27 2023, | https://doi.org/10.1111/nph.19435
The CARBON CATABOLITE REPRESSION 4A-mediated RNA deadenylation pathway acts on the transposon RNAs that are not regulated by small RNAs
Ling Wang1,2*, Hui Li1,2*, Zhen Lei1,2, Dong-Hoon Jeong3,4 and Jungnam Cho5
1National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiologyand Ecology, Chinese Academy ofSciences, Shanghai, 200032, China;
2University of Chinese Academy of Science, Beijing, 100049, China;
3Department of Life Science, Hallym University, Chuncheon, 24252, Korea;
4Multidisciplinary Genome Institute, Hallym University, Chuncheon, 24252, Korea;
5Department of Biosciences, Durham University, Durham, DH1 3LE, UK
*These authors contributed equally to this work.
Author for correspondence: Jungnam Cho
Abstract
Transposable elements (TEs) are mobile genetic elements that can impair the host genome stability and integrity. It has been well documented that activated transposons in plants are suppressed by small interfering (si) RNAs. However, transposon repression by the cytoplasmic RNA surveillance system is unknown. Here, we show that mRNA deadenylation is critical for controlling transposons in Arabidopsis. Trimming of poly(A) tail is a rate-limiting step that precedes the RNA decay and is primarily mediated by the CARBON CATABOLITE REPRESSION 4 (CCR4)-NEGATIVE ON TATA-LESS (NOT) complex. We found that the loss of CCR4a leads to strong derepression and mobilization of TEs in Arabidopsis. Intriguingly, CCR4a regulates a largely distinct set of TEs from those controlled by RNA-dependent RNA Polymerase 6 (RDR6), a key enzyme that produces cytoplasmic siRNAs. This indicates that the cytoplasmic RNA quality control mechanism targets the TEs that are poorly recognized by the previously well-characterized RDR6-mediated pathway, and thereby augments the host genome stability. Our study suggests a hitherto unknown mechanism for transposon repression mediated by RNA deadenylation and unveils a complex nature of the host's strategy to maintain the genome integrity.
논문정보
- Research article
- 2023년 11월 (BRIC 등록일 2023-11-28)
- 국내+국외 연구진
- 생명과학 > 식물학
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