구만복 (고려대학교)

한빛사논문

조회372

고려대학교

CV File 125 kb

Biosens. Bioelectron., Nov 20 2023, 246 S0956-5663(23)00785-6 | https://doi.org/10.1016/j.bios.2023.115843 Group selective aptamers: Broad-spectrum recognition of target groups in Cronobacter species and implementation of electrochemical biosensors as receptors

Hye Ri Kim ^a, Cheulmin Joe ^b, Ee Taek Hwang ^c, Man Bock Gu ^b, Byoung Chan Kim ^a,d

^aCenter for Sustainable Environment Research, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul, 02792, Republic of Korea

^bDepartment of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea

^cDepartment of Food Biotechnology, Dong-A University, Busan, Republic of Korea

^dDivision of Energy and Environment Technology, KIST School, University of Science and Technology (UST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul, 02792, Republic of Korea

Corresponding authors : Man Bock Gu, Byoung Chan Kim

Abstract

Aptamers are a versatile class of receptors with a high affinity and selectivity for specific targets. Although their ability to recognize individual targets has been extensively studied, some scenarios require the development of receptors capable of identifying all target groups. This study investigated the use of aptamers to achieve the broad-spectrum recognition of groups instead of individual targets. Aptamers were screened for selectively distinct groups of Cronobacter species associated with foodborne diseases. Seven Cronobacter spp. were divided into Group A (C. sakazakii, C. malonaticus, C. turicensis, and C. muytjensii) and Group B (C. dublinensis, C. condimenti, and C. universalis). Aptamers with exclusive selectivity for each group were identified, allowing binding to the species within their designated group while excluding those from the other group. The screened aptamers demonstrated reliable affinity and specificity with dissociation constants ranging from 1.3 to 399.7 nM for Group A and 4.0-24.5 nM for Group B. These aptamers have also been successfully employed as receptors in an electrochemical biosensor platform, enabling the selective detection of each group based on the corresponding aptamer (limit of detection was 7.8 and 3.2 CFU for Group A and Group B, respectively). The electrochemical sensor effectively detected the extent of infection in each group in powdered infant formula samples. This study highlights the successful screening and application of group-selective aptamers as sensing receptors, emphasizing their potential for diverse applications in different fields such as food safety, environmental monitoring, and clinical diagnostics, where the selective biosensing of target groups is crucial.

논문정보

형식Research article
게재일2023년 11월 (BRIC 등록일 2023-11-27)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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