김승일 (울산대학교 의과대학, 서울아산병원) | 한빛사논문 > 한빛사

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조회709

김승일Seungil Kim

울산대학교 의과대학, 서울아산병원

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Microbiome, 23 November 2023 | https://doi.org/10.1186/s40168-023-01687-8 Newly isolated Lactobacillus paracasei strain modulates lung immunity and improves the capacity to cope with influenza virus infection

Seungil Kim ^1,2, Sohyeon Lee ¹, Tae‑Young Kim ¹, Su‑Hyun Lee ¹, Sang‑Uk Seo ³ and Mi‑Na Kweon ^1,2,*

¹Mucosal Immunology Laboratory, Department of Convergence Medicine, University of Ulsan College of Medicine/Asan Medical Center, Seoul, Republic of Korea.
²Digestive Diseases Research Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
³Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.

^*Corresponding author: correspondence to Mi‑Na Kweon

Abstract

Background

The modulation of immune responses by probiotics is crucial for local and systemic immunity. Recent studies have suggested a correlation between gut microbiota and lung immunity, known as the gut–lung axis. However, the evidence and mechanisms underlying this axis remain elusive.

Results

In this study, we screened various Lactobacillus (L.) strains for their ability to augment type I interferon (IFN-I) signaling using an IFN-α/β reporter cell line. We identified L. paracasei (MI29) from the feces of healthy volunteers, which showed enhanced IFN-I signaling in vitro. Oral administration of the MI29 strain to wild-type B6 mice for 2 weeks resulted in increased expression of IFN-stimulated genes and pro-inflammatory cytokines in the lungs. We found that MI29-treated mice had significantly increased numbers of CD11c⁺PDCA-1⁺ plasmacytoid dendritic cells and Ly6C^hi monocytes in the lungs compared with control groups. Pre-treatment with MI29 for 2 weeks resulted in less weight loss and lower viral loads in the lung after a sub-lethal dose of influenza virus infection. Interestingly, IFNAR1^−/− mice did not show enhanced viral resistance in response to oral MI29 administration. Furthermore, metabolic profiles of MI29-treated mice revealed changes in fatty acid metabolism, with MI29-derived fatty acids contributing to host defense in a Gpr40/120-dependent manner.

Conclusions

These findings suggest that the newly isolated MI29 strain can activate host defense immunity and prevent infections caused by the influenza virus through the gut–lung axis.

논문정보

형식Research article
게재일2023년 11월 (BRIC 등록일 2023-11-24)
연구진국내 연구진
분야 의약학 > 면역의학

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