김도형 (University of Minnesota) | 한빛사논문 > 한빛사

한빛사논문

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University of Minnesota

Autophagy, Published online: 22 Nov 2023| https://doi.org/10.1080/15548627.2023.2286078 ULK1 seen at the single-molecule level during autophagy initiation

Chiranjib Banerjee ¹, Elias M Puchner ^{1 *}, Do-Hyung Kim ^{2 *}

¹School of Physics and Astronomy, University of Minnesota, Twin Cities.
²Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Twin Cities.

^*Corresponding authors: correspondence to Elias M Puchner or Do-Hyung Kim

Abstract

Macroautophagy/autophagy research often involves overexpressing proteins to investigate their localization, function and activity. However, this approach can disturb the inherent balance of cellular components, potentially affecting the integrity of the autophagy process. With the advent of genome-editing techniques like CRISPR-Cas9, it is now possible to tag endogenous proteins with fluorescent markers, enabling the study of their behaviors under more physiologically relevant conditions. Nevertheless, conventional microscopy methods have limitations in characterizing the behaviors of proteins expressed at endogenous levels. This challenge can be overcome by single-molecule localization microscopy (SMLM) methods, which provide single-molecule sensitivity and super-resolution imaging capabilities. In our recent study, we used SMLM in combination with genome editing to explore the behavior of endogenous ULK1 during autophagy initiation, yielding unprecedented insights into the autophagy initiation process.

논문정보

형식Research article
게재일2023년 11월 (BRIC 등록일 2023-12-18)
연구진국외 연구진
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