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Small, Nov 15 2023, | https://doi.org/10.1002/smll.202306793 Tailed-Hoogsteen Triplex DNA Silver Nanoclusters Emit Red Fluorescence upon Target miRNA Sensing

Hari Chandana Yadavalli ¹, Sooyeon Park ¹, Yeolhoe Kim ¹, Riddhi Nagda ¹, Tae-Hwan Kim ², Min Kyun Han ¹, Il Lae Jung ³, Yong Joo Bhang ⁴, Won Ho Yang ¹, Louise Torp Dalgaard ⁵, Seong Wook Yang ¹, Pratik Shah⁵

¹Department of Systems Biology, Institute of Life Science and Biotechnology, Yonsei University, Seoul, 03722, Republic of Korea.

²Department of Quantum System Engineering, Jeonbuk National University, Jeonju, 54896, Republic of Korea.

³Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, Daejeon, 34057, Republic of Korea.

⁴Xenohelix Research Institute, BT Centre 305, 56 Songdogwahak-ro Yeonsugu, Incheon, 21984, Republic of Korea.

⁵Department of Science and Environment, Roskilde University, Roskilde, 4000, Denmark.

H.C.Y. and S.P. contributed equally to this work.

CORRESPONDING AUTHORS: Seong Wook Yang, Pratik Shah

Abstract

MicroRNAs (miRNAs) are small RNA molecules, typically 21-22 nucleotides in size, which play a crucial role in regulating gene expression in most eukaryotes. Their significance in various biological processes and disease pathogenesis has led to considerable interest in their potential as biomarkers for diagnosis and therapeutic applications. In this study, a novel method for sensing target miRNAs using Tailed-Hoogsteen triplex DNA-encapsulated Silver Nanoclusters (DNA/AgNCs) is introduced. Upon hybridization of a miRNA with the tail, the Tailed-Hoogsteen triplex DNA/AgNCs exhibit a pronounced red fluorescence, effectively turning on the signal. It is successfully demonstrated that this miRNA sensor not only recognized target miRNAs in total RNA extracted from cells but also visualized target miRNAs when introduced into live cells, highlighting the advantages of the turn-on mechanism. Furthermore, through gel-fluorescence assays and small-angle X-ray scattering (SAXS) analysis, the turn-on mechanism is elucidated, revealing that the Tailed-Hoogsteen triplex DNA/AgNCs undergo a structural transition from a monomer to a dimer upon sensing the target miRNA. Overall, the findings suggest that Tailed-Hoogsteen triplex DNA/AgNCs hold great promise as practical sensors for small RNAs in both in vitro and cell imaging applications.

논문정보

형식Research article
게재일2023년 11월 (BRIC 등록일 2023-11-23)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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