박홍근 (Harvard University)

한빛사논문

조회288

Harvard University

Nat. Commun., 21 November 2023 | https://doi.org/10.1038/s41467-023-43333-9 A semiconductor 96-microplate platform for electrical-imaging based high-throughput phenotypic screening

Shalaka Chitale ¹, Wenxuan Wu ^1,2, Avik Mukherjee ³, Herbert Lannon ¹, Pooja Suresh ¹, Ishan Nag ¹, Christina M. Ambrosi ¹, Rona S. Gertner ⁴, Hendrick Melo ¹, Brendan Powers ¹, Hollin Wilkins ¹, Henry Hinton ², Michael Cheah ¹, Zachariah G. Boynton ¹, Alexander Alexeyev ¹, Duane Sword ¹, Markus Basan ³, Hongkun Park ^4,5,*, Donhee Ham ^2,* & Jeffrey Abbott ^1,2,4,5,*

¹CytoTronics Inc., Boston, MA, USA.
²John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA.
³Department of System Biology, Harvard Medical School, Boston, MA, USA.
⁴Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.
⁵Department of Physics, Harvard University, Cambridge, MA, USA.

^*Corresponding authors: correspondence to Hongkun Park, Donhee Ham or Jeffrey Abbott

Abstract

High-content imaging for compound and genetic profiling is popular for drug discovery but limited to endpoint images of fixed cells. Conversely, electronic-based devices offer label-free, live cell functional information but suffer from limited spatial resolution or throughput. Here, we introduce a semiconductor 96-microplate platform for high-resolution, real-time impedance imaging. Each well features 4096 electrodes at 25 µm spatial resolution and a miniaturized data interface allows 8× parallel plate operation (768 total wells) for increased throughput. Electric field impedance measurements capture >20 parameter images including cell barrier, attachment, flatness, and motility every 15 min during experiments. We apply this technology to characterize 16 cell types, from primary epithelial to suspension cells, and quantify heterogeneity in mixed co-cultures. Screening 904 compounds across 13 semiconductor microplates reveals 25 distinct responses, demonstrating the platform’s potential for mechanism of action profiling. The scalability and translatability of this semiconductor platform expands high-throughput mechanism of action profiling and phenotypic drug discovery applications.

논문정보

형식Research article
게재일2023년 11월 (BRIC 등록일 2023-12-04)
연구진국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

댓글 작성 로그인

CV 열람하기

연락처 보기