한상학 (한림대학교 의과대학)

한빛사논문

조회339

한상학Sang Hak Han

한림대학교 의과대학

Gut Microbes, Dec 2023, 15 (2) | https://doi.org/10.1080/19490976.2023.2281014 Gut-microbiota prompt activation of natural killer cell on alcoholic liver disease

Jung A Eom^a†, Jin-Ju Jeong^a†, Sang Hak Han^b†, Goo Hyun Kwon^a, Kyeong Jin Lee^a, Haripriya Gupta^a, Satya Priya Sharma^a, Sung-Min Won^a, Ki-Kwang Oh^a, Sang Jun Yoon^a, Hyun Chae Joung^c, Kyung Hwan Kim^c, Dong Joon Kim^a, and Ki Tae Suk^a

^aInstitute for Liver and Digestive Diseases, Hallym University, Chuncheon, Republic of Korea;

^bDepartment of Pathology, Hallym University College of Medicine, Chuncheon, Republic of Korea;

^cChong Kun Dang Bio Research Institute, Ansan-si, Gyeonggi-do, Republic of Korea

^†These authors equally contribute.

CONTACT: Ki Tae Suk

Abstract

The liver is rich in innate immune cells, such as natural killer (NK) cells, natural killer T cells, and Kupffer cells associated with the gut microbiome. These immune cells are dysfunctional owing to alcohol consumption. However, there is insufficient data on the association between immune cells and gut microbiome in alcoholic liver disease (ALD). Therefore, the purpose of this study was to evaluate the effects of probiotic strains on NK cells in ALD patients. In total, 125 human blood samples [control ( n = 22), alcoholic hepatitis ( n = 43), and alcoholic cirrhosis ( n = 60]) were collected for flow cytometric analysis. C57BL/6J mice were divided into four groups (normal, EtOH-fed, and 2 EtOH+strain groups [ Phocaeicola dorei and Lactobacillus helveticus]). Lymphocytes isolated from mouse livers were analyzed using flow cytometry. The frequency of NK cells increased in patients with alcoholic hepatitis and decreased in patients with alcoholic cirrhosis. The expression of NKp46, an NK cell-activating receptor, was decreased in patients with alcoholic hepatitis and increased in patients with alcoholic cirrhosis compared to that in the control group. The number of cytotoxic CD56dimCD16 ⁺ NK cells was significantly reduced in patients with alcoholic cirrhosis. We tested the effect of oral administration P. dorei and L. helveticus in EtOH-fed mice. P. dorei and L. helveticus improved liver inflammation and intestinal barrier damage caused by EtOH supply and increased NK cell activity. Therefore, these observations suggest that the gut microbiome may ameliorate ALD by regulating immune cells.

논문정보

형식Research article
게재일2023년 12월 (BRIC 등록일 2023-11-22)
연구진국내 연구진
분야 의약학 > 응용의학

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