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Sci. Data, Nov 16 2023, 10 (1) 804 | https://doi.org/10.1038/s41597-023-02727-6
Transcriptome profiles of Streptomyces clavuligerus strains producing different titers of clavulanic acid
Junpyo Gong1, Jeong Sang Yi1, Hang Su Cho1, Chang Hun Shin2, Hyung-Jin Won2, Byung-Kwan Cho3, Minsoo Noh1 & Yeo Joon Yoon1
1Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul, 08826, Republic of Korea.
2Research Institute of CKD BiO, Ansan, 15604, Republic of Korea.
3Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, 34141, Republic of Korea.
J.G. and J.S.Y. have contributed equally to this work.
Corresponding authors : Correspondence to Minsoo Noh or Yeo Joon Yoon.
Abstract
Streptomyces clavuligerus NRRL 3585 is a native producer of clavulanic acid (CA), a clinically used β-lactamase inhibitor, and is widely used as an industrial strain for the production of antibiotics. Selective random mutagenesis has successfully generated the improved CA-producing S. clavuligerus mutant strains as well as the strain with the loss of CA biosynthesis. To understand the molecular mechanisms associated with the improved CA-production potential, genome-scale RNA-sequencing-based transcriptional data were obtained for the wild-type S. clavuligerus strain and its three mutant strains. Total RNA samples for each strain were collected across four different growth stages, and all 32 sequencing data points exhibited an average Phred score of 36. The high-quality genome-scale transcriptional profile of S. clavuligerus strains with varied CA biosynthetic potential provides valuable insights and new opportunities for discovering efficient metabolic engineering strategies for the development of improved industrial strains.
논문정보
- Research article
- 2023년 11월 (BRIC 등록일 2023-11-20)
- 국내 연구진
- 생명과학 > 생화학
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