김륜형 (연세대학교)

한빛사 논문

조회68

연세대학교

Small, Nov 14 2023, | https://doi.org/10.1002/smll.202307262 Colorimetric Detection of HER2-Overexpressing-Cancer-Derived Exosomes in Mouse Urine Using Magnetic-Polydiacetylene Nanoparticles

Ryunhyung Kim ¹, Byeonggeol Mun ¹, Seongjae Lim ¹, Chaewon Park ¹, Jinyoung Kim ¹, Jaewook Lim ¹, Hyein Jeong ¹, Hye Young Son ², Hyun Wook Rho ², Eun-Kyung Lim^3,4,5, Seungjoo Haam ¹

¹Department of Chemical and Biomolecular Engineering, College of Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.

²Department of Radiology, College of Medicine, Yonsei University, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.

³Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.

⁴Department of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34113, Republic of Korea.

⁵School of Pharmacy, Sungkyunkwan University, Suwon, 16419, Republic of Korea.

CORRESPONDING AUTHORS : Eun-Kyung Lim, Seungjoo Haam

Abstract

Breast cancer (BC) is a major global health problem, with ≈20-25% of patients overexpressing human epidermal growth factor receptor 2 (HER2), an aggressive marker, yet access to early detection and treatment varies across countries. A low-cost, equipment-free, and easy-to-use polydiacetylene (PDA)-based colorimetric sensor is developed for HER2-overexpressing cancer detection, designed for use in low- and middle-income countries (LMICs). PDA nanoparticles are first prepared through thin-film hydration. Subsequently, hydrophilic magnetic nanoparticles and HER2 antibodies are sequentially conjugated to them. The synthesized HER2-MPDA can be concentrated and separated by a magnetic field while inheriting the optical characteristics of PDA. The specific binding of HER2 antibody in HER2-MPDA to HER2 receptor in HER2-overexpressing exosomes causes a blue-to-red color change by altering the molecular structure of the PDA backbone. This colorimetric sensor can simultaneously separate and detect HER2-overexpressing exosomes. HER2-MPDA can detect HER2-overexpressing exosomes in the culture medium of HER2-overexpressing BC cells and in mouse urine samples from a HER2-overexpressing BC mouse model. It can selectively isolate and detect only HER2-overexpressing exosomes through magnetic separation, and its detection limit is found to be 8.5 × 10 ⁸ particles mL ^-1 . This colorimetric sensor can be used for point-of-care diagnosis of HER2-overexpressing BC in LMICs.

논문정보

형식Research article
게재일2023년 11월 (BRIC 등록일 2023-11-15)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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