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POSTECH

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Adv. Mater., Oct 2023, 35 (42) | https://doi.org/10.1002/adma.202303655 G-Quadruplex-Filtered Selective Ion-to-Ion Current Amplification for Non-Invasive Ion Monitoring in Real Time

Hyebin Yoo ¹, Hyun-Ro Lee ¹, Soon-Bo Kang ², Juhwa Lee ³, Kunwoong Park ⁴, Hyunjae Yoo ², Jinmin Kim ¹, Taek Dong Chung ⁵, Kyung-Mi Lee ⁶, Hyun-Ho Lim ⁴, Chang Yun Son^3,7, Jeong-Yun Sun^2,8, Seung Soo Oh ^1,7

¹Department of Materials Science & Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, 37673, South Korea.

²Department of Materials Science & Engineering, Seoul National University, Seoul, 08826, South Korea.

³Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, 37673, South Korea.

⁴Neurovascular Unit Research Group, Korea Brain Research Institute (KBRI), Daegu, 41062, South Korea.

⁵Department of Chemistry, Seoul National University, Seoul, 08826, South Korea.

⁶Department of Biochemistry and Molecular Biology, Korea University College of Medicine, Seoul, 02841, South Korea.

⁷Institute for Convergence Research and Education in Advanced Technology (I-CREATE), Yonsei University, Incheon, 21983, South Korea.

⁸Research Institute of Advanced Materials (RIAM), Seoul National University, Seoul, 08826, South Korea.

CORRESPONDING AUTHORS: Hyun-Ho Lim, Chang Yun Son, Jeong-Yun Sun, Seung Soo Oh

Abstract

Living cells efflux intracellular ions for maintaining cellular life, so intravital measurements of specific ion signals are of significant importance for studying cellular functions and pharmacokinetics. In this work, de novo synthesis of artificial K ⁺ -selective membrane and its integration with polyelectrolyte hydrogel-based open-junction ionic diode (OJID) is demonstrated, achieving a real-time K ⁺ -selective ion-to-ion current amplification in complex bioenvironments. By mimicking biological K ⁺ channels and nerve impulse transmitters, in-line K ⁺ -binding G-quartets are introduced across freestanding lipid bilayers by G-specific hexylation of monolithic G-quadruplex, and the pre-filtered K ⁺ flow is directly converted to amplified ionic currents by the OJID with a fast response time at 100 ms intervals. By the synergistic combination of charge repulsion, sieving, and ion recognition, the synthetic membrane allows K ⁺ transport exclusively without water leakage; it is 250× and 17× more permeable toward K ⁺ than monovalent anion, Cl ^- , and polyatomic cation, N-methyl-d-glucamine ⁺ , respectively. The molecular recognition-mediated ion channeling provides a 500% larger signal for K ⁺ as compared to Li ⁺ (0.6× smaller than K ⁺ ) despite the same valence. Using the miniaturized device, non-invasive, direct, and real-time K ⁺ efflux monitoring from living cell spheroids is achieved with minimal crosstalk, specifically in identifying osmotic shock-induced necrosis and drug-antidote dynamics.

논문정보

형식Research article
게재일2023년 10월 (BRIC 등록일 2023-11-08)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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