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한국화학연구원

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Bioresour. Technol., Nov 04 2023, S0960-8524(23)01348-2 | https://doi.org/10.1016/j.biortech.2023.129920 Engineering of Yarrowia lipolytica as a platform strain for producing adipic acid from renewable resource

Sang Min Lee ^a, Ju Young Lee ^a, Ji-Sook Hahn ^b, Seung-Ho Baek^a

^aCenter for Bio-based Chemistry, Korea Research Institute of Chemical Technology (KRICT), Ulsan 44429, Republic of Korea

^bSchool of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Republic of Korea

Corresponding author : Seung-Ho Baek

Abstract

There is an increasing demand for bio-based dicarboxylic acids (DCA) as an eco-friendly alternatives to chemically synthesized DCA. Adipic acid, which is not naturally produced by microorganisms, is an essential DCA with significant industrial importance. This study aimed to develop a platform strain using Yarrowia lipolytica for efficient bioconversion of renewable resources into adipic acid. To prevent the complete oxidation of adipic acid, peroxisomal β-oxidation was engineered by selectively disrupting acyl-CoA oxidases. Furthermore, ω-oxidation activity was improved via introducing an additional copy of cytochrome P450 monooxygenase (ALK5) and reductase (CPR1) with fatty alcohol oxidase (FAO1). The production phase used SP92D medium in a two-stage bioconversion process, during which the engineered strain exhibited the highest production level, achieving a remarkable 9.7-fold increase compared to that of the parental strain. To our knowledge, this is the first report demonstrating that engineered Y. lipolytica can produce adipic acid from fatty acid methyl esters.

논문정보

형식Research article
게재일2023년 11월 (BRIC 등록일 2023-11-08)
연구진국내 연구진
분야 생명과학 > 생물공학

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