성민동 (연세대학교 의과대학) | 한빛사논문 > 한빛사

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조회541

성민동MinDong Sung

연세대학교 의과대학

Light-Sci. Appl., Nov 07 2023, 12 (1) | https://doi.org/10.1038/s41377-023-01309-w Three-dimensional label-free morphology of CD8 + T cells as a sepsis biomarker

MinDong Sung¹, Jong Hyun Kim², Hyun-Seok Min³, Sooyoung Jang², JaeSeong Hong², Bo Kyu Choi², JuHye Shin¹, Kyung Soo Chung¹ and Yu Rang Park²

¹Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Republic of Korea

²Department of Biomedical Systems Informatics, Yonsei University College of Medicine, Seoul, Republic of Korea

These authors contributed equally: MinDong Sung, Jong Hyun Kim, Hyun-Seok Min

Corresponding authors : Correspondence to Kyung Soo Chung or Yu Rang Park.

Abstract

Sepsis is a dysregulated immune response to infection that leads to organ dysfunction and is associated with a high incidence and mortality rate. The lack of reliable biomarkers for diagnosing and prognosis of sepsis is a major challenge in its management. We aimed to investigate the potential of three-dimensional label-free CD8 + T cell morphology as a biomarker for sepsis. This study included three-time points in the sepsis recovery cohort (N = 8) and healthy controls (N = 20). Morphological features and spatial distribution within cells were compared among the patients' statuses. We developed a deep learning model to predict the diagnosis and prognosis of sepsis using the internal cell morphology. Correlation between the morphological features and clinical indices were analysed. Cell morphological features and spatial distribution differed significantly between patients with sepsis and healthy controls and between the survival and non-survival groups. The model for predicting the diagnosis and prognosis of sepsis showed an area under the receiver operating characteristic curve of nearly 100% with only a few cells, and a strong correlation between the morphological features and clinical indices was observed. Our study highlights the potential of three-dimensional label-free CD8 + T cell morphology as a promising biomarker for sepsis. This approach is rapid, requires a minimum amount of blood samples, and has the potential to provide valuable information for the early diagnosis and prognosis of sepsis.

논문정보

형식Research article
게재일2023년 11월 (BRIC 등록일 2023-11-07)
연구진국내 연구진
분야 의약학 > 면역의학

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