전진 (충남대학교)

한빛사논문

조회430

충남대학교

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J. Adv. Res., Nov 03 2023, S2090-1232(23)00324-7 | https://doi.org/10.1016/j.jare.2023.11.001 Modulation of Warm Temperature-sensitive Growth using a Phytochrome B Dark Reversion Variant, phyB[G515E], in Arabidopsis and Rice

Jin Jeon ^a, Md Mizanor Rahman ^b, Hee Wook Yang ^a, Jaewook Kim ^a, Ho-Jun Gam ^c, Ji Young Song ^a, Seok Won Jeong ^a, Jeong-Il Kim ^d, Myoung-Goo Choi ^e, Dong-Ho Shin ^a, Giltsu Choi ^f, Donghwan Shim ^a, Jae-Hoon Jung ^g, In-Jung Lee ^c, Jong Seong Jeon ^b, Youn-Il Park ^a

^aDepartment of Biological Sciences, Chungnam National University, Daejeon 34134, Korea

^bGraduate School of Green-Bio Science and Crop Biotech Institute, Kyung Hee University, Yongin 17104, Korea

^cDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea

^dDepartment of Molecular Biotechnology and Kumho Life Science Laboratory, Chonnam National University, Gwangju 61186, Korea

^eNational Institute of Crop Science, Rural Development Administration, Wanju 55365, Korea

^fDepartment of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea

^gDepartment of Biological Sciences, Sungkyunkwan University, Suwon 16419, Korea

Corresponding authors : Jong Seong Jeon, Youn-Il Park

Abstract

Introduction: Ambient temperature-induced hypocotyl elongation in Arabidopsis seedlings is sensed by the epidermis-localized phytochrome B (phyB) and transduced into auxin biosynthesis via a basic helix-loop-helix transcription factor, phytochrome-interacting factor 4 (PIF4). Once synthesized, auxin travels down from the cotyledons to the hypocotyl, triggering hypocotyl cell elongation. Thus, the phyB-PIF4 module involved in thermosensing and signal transduction is a potential genetic target for engineering warm temperature-insensitive plants.

Objectives: This study aims to manipulate warm temperature-induced elongation of plants at the post-translational level using phyB variants with dark reversion, the expression of which is subjected to heat stress.

Methods: The thermosensitive growth response of Arabidopsis was manipulated by expressing the single amino acid substitution variant of phyB (phyB[G515E]), which exhibited a lower dark reversion rate than wild-type phyB. Other variants with slow (phyB[G565E]) or rapid (phyB[S584F]) dark reversion or light insensitivity (phyB[G767R]) were also included in this study for comparison. Warming-induced transient expression of phyB variants was achieved using heat shock-inducible promoters. Arabidopsis PHYB[G515E] and PHYB[G565E] were also constitutively expressed in rice in an attempt to manipulate the heat sensitivity of a monocotyledonous plant species.

Results: At an elevated temperature, Arabidopsis seedlings transiently expressing PHYB[G515E] under the control of a heat shock-inducible promoter exhibited shorter hypocotyls than those expressing PHYB and other PHYB variant genes. This warm temperature-insensitive growth was related to the lowered PIF4 and auxin responses. In addition, transgenic rice seedlings expressing Arabidopsis PHYB[G515E] and PHYB[G564E] showed warm temperature-insensitive shoot growth.

Conclusion: Transient expression of phyB variants with altered dark reversion rates could serve as an effective optogenetic technique for manipulating PIF4-auxin-mediated thermomorphogenic responses in plants.

논문정보

형식Research article
게재일2023년 11월 (BRIC 등록일 2023-11-06)
연구진국내 연구진
분야 생명과학 > 식물학

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