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고려대학교

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Bioresour. Technol., Nov 02 2023, S0960-8524(23)01391-3 | https://doi.org/10.1016/j.biortech.2023.129963 Biosynthesis of l-histidine from marine biomass-derived galactans in metabolically engineered Corynebacterium glutamicum

Minhye Kim ^a,1, Jun Won Oh ^a,1, Da Woon Jeong^a,c, Byeong-Hyeon Cho ^a, Joonhee Chang ^a, Xiaoyu Shi ^a, Sung Ok Han ^a,b

^aDepartment of Biotechnology, Korea University, Seoul 02841, Republic of Korea

^bInstitute of Life Science and Natural Resources, Korea University, Seoul 02841, Republic of Korea

^cAmtixbio CO., LTD., Seoul 01411, Republic of Korea

¹These authors equally contributed to this paper.

Corresponding author: Sung Ok Han

Abstract

l-Histidine plays significant roles in the food and pharmaceutical industries, and its demand has been steadily increasing recently. As demand for l-histidine continues, the development of eco-friendly processes is required. To pursue this goal, D-galactose, a primary component of red algae, was employed as a carbon source for synthesizing l-histidine. To harness this marine biomass, κ-carrageenan was preferentially hydrolyzed to obtain D-galactose using κ-carrageenase (CgkA) and iduronate-2-sulfatase (IdsA3). Subsequently, l-histidine production was enhanced by modifying precursor pathways in Corynebacterium glutamicum. The resulting strain, TDPH6 exhibited a remarkable 2.15-fold increase in l-histidine production compared to TDP. Furthermore, a galactose utilization system was introduced and named TDPH6G2. During fermentation, this strain efficiently consumed 100 % of the D-galactose and synthesized 0.395 g/L of l-histidine. In conclusion, this study presents a sustainable approach to L-histidine synthesis by introducing a galactose utilization system into C. glutamicum.

논문정보

형식Research article
게재일2023년 11월 (BRIC 등록일 2023-11-06)
연구진국내 연구진
분야 생명과학 > 생물공학

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