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조회497

김영훈Young Hun Kim

아주대학교

J. Control. Release, Volume 364, December 2023, Pages 420-434 | https://doi.org/10.1016/j.jconrel.2023.10.047 In-situ wound healing by SDF-1-mimic peptide-loaded click crosslinked hyaluronic acid scaffold

Young Hun Kim^a, Shina Kim ^a, Hyun Jin Ju ^a, Min Ji Han ^a, Yongdoo Park ^b, Eunha Kim ^a, Hak Soo Choi ^c, Sangdun Choi ^a, Moon Suk Kim^a,d

^aDepartment of Molecular Science and Technology, Ajou University, Suwon 16499, Republic of Korea

^bDepartment of Biomedical Sciences, College of Medicine, Korea University, Seoul 02841, Republic of Korea

^cGordon Center for Medical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA

^dResearch Institute, Medipolymer, Woncheon Dong 332-2, Suwon 16522, Republic of Korea

Corresponding author : Moon Suk Kim

Abstract

Endogenous stem cell-based in-situ tissue regeneration has recently gained considerable attention. In this study, we investigated the potential of a chemokine, SDF-1-mimic peptide (SMP), to promote endogenous stem cell-based in-situ wound healing. Our approach involved the development of a click crosslinked hyaluronic acid scaffold loaded with SMP (Cx-HA + SMP) to release SMP in a wound site. The Cx-HA scaffold maintained its structural integrity throughout the wound healing process and also captured endogenous stem cells. Gradual SMP release from the Cx-HA + SMP scaffold established a concentration gradient at the wound site. In animal wound experiments, Cx-HA + SMP exhibited faster wound contraction compared to Cx-HA + SDF-1. Additionally, Cx-HA + SMP resulted in approximately 1.2-1.6 times higher collagen formation compared to Cx-HA + SDF-1. SMP released from the Cx-HA + SMP scaffold promoted endogenous stem cell migration to the wound site 1.5 times more effectively than Cx-HA + SDF-1. Moreover, compared to Cx-HA + SDF-1, Cx-HA + SMP exhibited higher expression of CXCR4 and CD31, as well as the positive markers CD29 and CD44 for endogenous stem cells. The endogenous stem cells that migrated through Cx-HA + SMP regenerated into wound skin with minimal scar granule formation, similar to the normal tissue. In conclusion, SMP peptide offers greater convenience, while efficiently attracting migrating endogenous stem cells compared to the SDF protein. Our findings suggest that Cx-HA + SMP scaffolds hold promise as a strategy to enhance endogenous stem cell-based in-situ wound healing.

논문정보

형식Research article
게재일2023년 12월 (BRIC 등록일 2023-11-09)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오소재

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