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University of California, San Diego

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Neuron, October 30, 2023 | https://doi.org/10.1016/j.neuron.2023.09.038 Viral vector-mediated transgene delivery with novel recombinase systems for targeting neuronal populations defined by multiple features

Minju Jeong,^1,7 Jun-Hyeok Choi,^1,7 Hyeonseok Jang,¹ Dong Hyun Sohn,² Qingdi Wang,¹ Joann Lee,¹ Li Yao,¹ Eun Ji Lee,¹ Jiachen Fan,¹ Marta Pratelli,¹ Eric H. Wang,¹ Christen N. Snyder,³ Xiao-yun Wang,¹ Sora Shin,^4,5 Aryn H. Gittis,³ Tsung Chang Sung,⁶ Nicholas C. Spitzer,¹ and Byung Kook Lim^1,8,*

¹Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA
²Department of Microbiology and Immunology, Pusan National University School of Medicine, Yangsan 50612, Republic of Korea
³Department of Biological Sciences and Center for the Neural Basis of Cognition, Carnegie Mellon University, Pittsburgh, PA 15213, USA
⁴Center for Neurobiology Research, Fralin Biomedical Research Institute at Virginia Tech Carilion, Virginia Tech, Roanoke, VA, USA
⁵Department of Human Nutrition, Foods, and Exercise, Virginia Tech, Blacksburg, VA, USA
⁶Transgenic Core, Salk Institute for Biological Studies, La Jolla, CA, USA
⁷These authors contributed equally
⁸Lead contact

^*Corresponding author: correspondence to Byung Kook Lim

Abstract

A comprehensive understanding of neuronal diversity and connectivity is essential for understanding the anatomical and cellular mechanisms that underlie functional contributions. With the advent of single-cell analysis, growing information regarding molecular profiles leads to the identification of more heterogeneous cell types. Therefore, the need for additional orthogonal recombinase systems is increasingly apparent, as heterogeneous tissues can be further partitioned into increasing numbers of specific cell types defined by multiple features. Critically, new recombinase systems should work together with pre-existing systems without cross-reactivity in vivo. Here, we introduce novel site-specific recombinase systems based on ΦC31 bacteriophage recombinase for labeling multiple cell types simultaneously and a novel viral strategy for versatile and robust intersectional expression of any transgene. Together, our system will help researchers specifically target different cell types with multiple features in the same animal.

논문정보

형식Research article
게재일2023년 10월 (BRIC 등록일 2023-10-31)
연구진국내+국외 연구진
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